Study On Proliferation And Invasion Of Cells In Colorectal Cancer Induced By Matrix M Eetalloproteinase 2

Colorectal cancer is one of the most common types of cancer in worldwide,, it is a great threat to human health. As in other malignant tumor,colorectal cancer has strong growth, invasion and metastasis with cells breakthrough the invasion of the surrounding matrix around the surrounding matrixand blood vessels, resulting in local or distant metastasis and blood transport.The study found that the process of tumor cells to extracellular matrix andbasement membrane damage plays an important role in cell penetration.Series of matrix metalloproteinases (MMPs) is an important enzymewhich can degrade the extracellular matrix. It can degradation of extracellularmatrix of almost all the ingredients,The expression of Matrix metalloproteinasein tumor cells is more than normal cells,and related studies have shown thatmatrix metalloproteinases can be adjusted by angiogenic factors such as VEGF,bFGF, TGF-É‘, to promote tumor angiogenesis.MMPs is a family of enzymesrelated closely to development, transfer and other biological processes of tumorcell. The biological characteristics also determine the matrix metalloproteinasefamily can become a new target for the treatment of cancer biology.In this investigation, we demonstrated that MMP-2 was predominantlyexpressed in the cancer cells and epithelial cells. And our experimentallyconfirmed that the MMP-2 in colorectal cancer tissues was significantly higherthan normal tissue, which also shows the level of MMP-2 is widely present incolorectal cancer in pathology.At the same time.MMP-2'expression werepositively correlated with tumor size, lymph node metastasis, local and distanttumor metastasis, tumor Duke's stage,and MMP-2 was highly expressed in thesurvival time of patients is far lower than low expression patients .MMPs overexpression has been intensively investigated as potential biomarkers andunfavorable factors in colorectal cancer.In further cytology test, we decreased MMP-2 expression in CRC celllines with lentiviral-mediated shRNA. Establish stably transfected colorectalcancer cell lines.We found that inhibition of MMP-2 expression couldmarkedly reduce the ability of cell proliferation and invasiveness in vitro.Furthermore, we found that the protein levels of VEGF and MT1-MMP weremarkedly reduced in MMP-2 suppressed colorectal cells compared to theircontrol cells, suggesting that MMP-2 can positively regulate the expression ofVEGF and MT1-MMP to promoting cell proliferation and invasion.In summary, our investigation demonstrated that the expression level ofMMP-2 was significantly increased in CRC. MMP-2'expression werepositively correlated with tumor size, tumor metastasis, tumor Duke's stage, wefound that reduce the expression of MMP-2 could cintrol the ability of cellgrowth and invasion. Finally, MMP-2 promotes CRC cell growth and invasionby increasing the expression VEGF and MT1-MMP. Thus we concluded that,inhibition of MMP-2 and can suppression tumor cell proliferation, growth incolorectal cancer,can provides a potential target for the treatment of colorectalcancer.