| ObjectiveChronic Fatigue Syndrome was named by DCD in1988. Now, the incidence of CFS is high, and higher and higher year by year. CFS seriously affects people's quality of life. Chinese Traditional Medicine in treatment of it has a certain advantage. We set up CFS rat model by chronic multiply factors to simulate the clinical manifestations of CFS and treated models by moxibustion. the experiment researched the effect and mechanism of therapy of moxibustion by observing the behavior, biochemical indexes and the content of the serum ACTH, CORT, CRH,IL-1β, IL-6of each group rats, to achieve an effective treatment method of chronic fatigue syndromeMethodWe divided50Wistar rats into the moxibustion group as the threrapy group, the normal group and the model group randomly. All rats were feeded a week, To set up CFS rat model, the rats of the model group and the moxibustion group were deprived sleep for20hours and forced to swim exhaustedly in eighth days, for3weeks. The normal group' rats were feeded normal without any stimulation. The next day of end of setting up model, the moxibustion group'rats were treated by moxibustion on the acupoints of Zusanli and Shenshu, Each acupoint was treated for eight minutes every day, five days a week, treated for three weeks continuously. The rats of the normal group and the model group weren't treated.We obeserved the behavior of each group rats before the setting up model and after and the end of treatment, and detected the biochemical indexes and the content of the serum ACTH, CORT,CRH.Result 1. The end of setting up model, the weigh, exhausting swimming time, rat tail suspension time, horizontal movement and vertical motion of the model group and moxibustion group'rats was lighter, shorter, longer, less than the normal group, the difference was statistically significant (P<0.05), which shows that the model rats appear the physical fatigue and mental fatigue. After the treatment, the weigh,exhausting swimming time,rat tail suspension time, horizontal movement and vertical motion of the moxibustion group'rats was heavier,longer, shorter, more than the model group, the difference was statistically significant (P<0.05), which shows that moxibustion on acupoints of Zusanli and Shenshu can improve model rats' physical strength, adjust their depression.2. After the treatment, the content of the serum ALT, AST, ALB, BUN, UA, LDH, CK of model group' rats were compared to the normal group, the difference was statistically significant(P<0.05). The content of the serum TP, GLB, CREAT, GLU, THCO, TG, CRP of model group' rats were compared to the normal group, the difference was'nt statistically significant (P>0.05), which shows that multiple factors may affect the biochemical indexes of CFS rats.3. The content of the serum ALT, AST, ALB, BUN, UA, LDH, CK of model group'rats were compared to the normal group, the difference was statistically significant (P<0.05). While the ALT and UA of both group's haven't significant difference.4. The content of the serum ACTH,CRH, CORT of the model group'rats was compared with the normal group, the difference was statistically significant (P<0.05), which shows that hypothalamus-pituitary-adrenergic axis'function of model rats is in condition of hyper.5. The content of the serum ACTH, CRH, CORT of the moxibustion group'rats was compared with the model group, the difference was statistically significant (P<0.05), which shows that moxibustion on the acupionts of Zusanli and Shenshu can regulate the function of the hypothalamus-pituitary adrenergic axis.6. The content of the serum IL-1β, IL-6of the moxibustion group'rats was compared with the model group, the difference was statistically significant (P<0.01), which shows that moxibustion on the acupionts of Zusanli and Shenshu can regulate the function of the immunecell IL-1β, IL-6. Conclusion1. We set up CFS rat model by multiple factors, After treatment, model rats'weigh was ligther, exhausting swimming time was shorter, rat tail suspension time was longer, horizontal movement and Vertical motion were less, which was similar to the clinical presentation of CFS, so, the setting up model was success.2. Moxibustion on the acupoints of Zusanli and Shenshu can improve the fatigue state of chronic fatigue rats significantly, recover certain biochemical indexes, so, which is an effective treatment of CFS.3. The hyperactivity of HPA axis and the change of immune system may be the pathogenesis of CFS.4. There is a close contact between immunecells and the hypothalamus-pituitary-adrenalaxis, and the nervors system, endocrine System, immune system interact.5. Moxibustion on zusanli and shenshu can improve HPA axis hyperactivity state, modulate the immune system, which suggests that the mechanism of moxibustion treatment for CFS is in many ways of regulating the whole.
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