Effect Of Tanshinone Iia On Cell Attachment Of Entopic Endometrium Cells In Endometriosis

Purpose:The en dometriosis sickness is one of Chinese medicine gynecology department difficult sickness. The mechanism of its development is not clear and no satisfactory treatment has been found currently. Currently most Chinese scholars think that blood stasis block is the main cause of the disease, for activating blood circulation and eliminating stasis its basic treatment method. Modern research proves endometrial can be grown in other cities, required to pass through "Attachment-Aggression-Angiogenesis" three pathological process, the mechanism of the molecular level research suggests, blocking one of the three, can affect uterus the formation and development. Eutopic endometrium is the key of endometriosis, the decision factors of endometriosis is the difference of Eutopic endometrium. Adhesion is the first step of endometriosis formed. So we choose to put the medicine in the Eutopic endometrium directly, researching the influence of the attachment effect with the medicine, and we hope that we can preventing this disease from the "source". This project had successful completing the culture of Eutopic endometrium cell of endometriosis, and putting Tanshinone ⅡA on the Eutopic endometrium, researching the influence of the attachment effect with Tanshinone ⅡA.Methods:1. Refer to method to culture cells of Ryan, we successfully established Eutopic endometrium cell of endometriosis in vitro model.2. We put different concentrations of Tanshinone IIA on the Eutopic endometrium cell of endometriosis, Using the western blot method to test protein content of expression of E-cadherin, CD44V6, ICAM-1.Result:1. This study confirmed that training of the two groups of different period of Eutopic endometrium had statistical significance. P<0.05.But training of the two groups of different ways of getting material of Eutopic endometrium had no statistical significance, P>0.05。2. Protein of E-cadherin, CD44V6, ICAM-1in Control group are high expression, comparing with this group, High dose group of Tanshinone IIA had lower expression, p<0.01; Middle dose group and low dose group of Tanshinone IIA had lowest expression, p<0.05.Conclusion:1. It is possible to establish Eutopic endometrium cell of endometriosis in vitro model.2. This study had proved that Tanshinone IIA can improve the Protein of E-cadherin, CD44V6, ICAM-1, Tanshinone IIA maybe stop the happen and the development of endometriosis.