The Study Of CD45RO,CD4,CD8,CD68,HLA-DRCD57 Expression In Endometriosis: Comparison Of Eutopic And Ectopic Endometrium With Normal Endometrium

Objective: Immunologic mechanisms are implicated in the pathogenesis of endometnosis. The purpose of this study was to assess CD45RO, CD4, CD8, CD68, HLA-DR, CD57 and T-lymphocyte, NK cells in eutopic and ectopic endometrium of endometnosis women and to explore the role and activity of. immunocyte in endometnosis, and to support the immune therapy.METHODS: Forty women with histopathological diagnosed endometriosis after operation at stage Ⅰ-Ⅳ according to the criteria by Revised American Fertility Society were selected as endometriosis group (10 were stage Ⅰ, 10 were stage Ⅱ, 20 were stage Ⅲ to Ⅳ) , eutopic endometrial samples were histologically divided into proliferative (n = 18) and secretory (n = 22) phases. Fifteen women with tubal ligation and reana stomosis of tubes were selected as control group, eutopic endometrial samples were histologically divided into proliferative (n = 8) and secretory (n = 7) phases. Ectopic endometrium obtained at operation from forty women with endometriosis were compared with eutopic endometrium from all women with and without endometriosis. Tissues were frozen in ultra low -70℃ immediately, then formalin-fixed, paraffin-embedded, sectioned and stained using the in situ hybridization technique to assess CD45RO,CD4, CD8, CD68, HLA-DR, CD57 in eutopic and ectopic endometrium.RESULTS: Expression of CD45RO, CD8 in T-lymphocyte and CD68 macrophages of eutopic and ectopic endometrium of women with endometriosis were significantly increased compared with that of the control figures P<0.05. They were significantly increased in macrophages of ectopic endometrium compared with eutopic endometrium P<0.05. CD4 T-lymphocyte increased in eutopic and ectopic endometrium compared with that of the control group, butthere was no significant difference(P>0.05). CD4/CD8 decreased compared with that of the control figures P<0.05. There was no significant difference(P>0.05) in CD4 and CD4/CD8, comparing with eutopic and ectopic endometrium. HLA-DR antigen positive in eutopic endometrium was a little higher than control group, but there was no significant difference(P>0.05). HLA-DR antigen positive in ectopic endometrium was higher than that in eutopic endometrium and control group, there all had significant difference P<0.05. There was no significant difference in CD57 NK among eutopic endometrium, ectopic endometrium and control group. CD45RCK CD4^ CD8, CD57 CD68 and HLA-DR antigen positive increased from proliferative phase to secretory phase , but there was no significant difference(P > 0.05). There was no significant difference for CD45RO,CD4,CDgi CD68 and HLA-DR antigen positive in ectopic endometrium from proliferative phase to secretory phase P>0.05. There was no significant difference in the number of CD45RO^ CD4, CD8 > CD57 > CD68 and HLA-DR antigen positive, comparing with the two cases of I - II stage and III-JV stage. P >0.05. The number of CD45RO,CD4,CD8, CD68 and HLA-DR antigen positive increased in the control group from proliferative phase to secretory phase, but there was no significant difference(P>0.05).CONCLUSIONS: The results of this observational study suggest that the number of activated macrophages were increased in ectopic and eutopic endometrium and the antigen submission effect of was visible in ectopic endometrium, T-lymphocyte relocation and immunocyte state changing all indicated the relationship between endometriosis and immunocyte. The relocation of macrophages and T-lymphocyte were the pre-condition of endometriosis, and the number and function changing of macrophages around the focus, the relocation of T-lymphocyte and the function changing of NK cells all effect the happening, progress and sustainment of endometriosis. The variety ofimmunocyte in ectopic and eutopic endometrium have no distinct relationship with catamenia cycle and disease stage.