| In recent years many research have proved that neural stem cells (NSCs) capable of self-renewal and multipotency persists in two adult mammalian brain regions: the subependymal zone (SVZ) of lateral ventricle and the subgranular zone (SGZ) in the hippocampal dentate gyrus. Neurotrophins such as basic fibroblast growth factor(bFGF) and brain-derived neurotrophic factor(BDNF) can promote the proliferation, migration and differentiation of NSCs, also have effects of on neuroprotection and neurorepair. We try to investigate the influences of cortical devasculating on the NSCs as well as the expressing of bFGF and BDNF in adult rat forebrain. Further we want to make sure the treatment effects of Panax Notoginseng saponins on it, and extend the foundation of modern medical science theories of the method of promoting tissue regeneration by removing blood stasis of Chinese medcine in treatment of brain disease.1 Influences of cortical devasculating on the NSCs in svz in adult rat and effects of Panax Notoginseng saponins on itOur research used adult Wistar rats with cortical devasculating on the left. BrdU was used ip to label the proliferating cells, and immunohistochemistry method was applicated to observe the influences of cortical devasculating on the proliferation, migration, and differentiation of NSCs and the expression of bFGF in SVZ,.In addition we observed the effects of Panax Notoginseng saponins on it. The results from serial sections showed that BrdU, PCNA, Nestin, Tuj-1 and Vimentin positive cells in SVZ from the caudal to the rostral of the lateral ventricle, including anterior horn, central part and inferior horn, were all increased. The reaction of them became stronger and the shape changed obviously after cortical devasculating. Tuj-1 mainly expressed in anterior horn of lateral ventricle, and glail cells were mainly located in the inferior horn of lateral ventricle. The expression of bFGF increased in SVZ at different level of the lateral ventricle, indicated that cortical devasculating induced the expression of it. bFGF might act on it's receptor by autocrine or paracrine, so that stimulated the quiescent NSCs in SVZ to proliferate and differentiate. After treatment of Panax Notoginseng saponins, the expressions of all above increased and strengthen, indicated Panax Notoginseng saponins might promote the proliferation and differentiation of NSCs in SVZ through the mechanism of increasing the expression of endogenous bFGF after cortical devasculating. 2 Cortical devasculating induced the neural stem cells in subependymal zone to proliferate and form migrating stream to the lesion siteCortical devasculating not only accelerated the proliferation of NSCs in SVZ and RMS, but also induced the proliferation of SVZ in inferior horn of lateral ventricle in adult rat. The proliferating cells formed two chains (H chain and L chai) of migrating stream, and migrated to the cortical lesion site through corpus callosum.The H chain derived from the upperoir wall of inferior horn, and the L chain derived from the lateral wall of inferior horn. Cells coming from the RMSalso participated in the L chain. Cell components of these two chains of migrating stream included GFAP positive mature astrocytes, Vimentin positive imature astrocytes, and Tuj-1 positive neuroblasts. These positive cells arrived the cortical lesion site and might contribute to regeneration and restoration. The H and the L chain also expressed bFGF and BDNF. These results indicated bFGF and BDNF might act on their receptors by autocrine or paracrine, and in turn not only accelerated the proliferation of SVZ, but also promoted the migration and differentiation of the H chain and the L chain. The H chain and the L chain arrived to lesion site participated in the repair. 3 Influences of cortical devasculating on the neural stem cells in hippocampal dentate gyrus in adult rat Our research observed the effects of cortical devasculating on the NSCs and the expression of bFGF in SGZ in adult rat. The results showed that the numbers of Br...
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