| Studies on the chemical constituents of Urtica Cannabina L. and the active parts of the Prescription for benefited Qi to Promote flow of blood.Urtica Cannabina L. is one species of Urtica. There are very rich plant resources in the northwest of China. The local people use U. cannabina to care rheumatism, nettle rash, wounds bited by snakes and so on. It was said to have the function of driving away wind-dampness, opening channels and collaterals, relaxing convulsions, antiinflammation and detumescence. While, Urtica dioica is another species of Urtica as same as U. Cannabina .It widely distributed in the Europe and had been used for a long time. From the 1960's to now, the foreign pharmaceutists had developed a lot of healthness protecting products and OTC drugs. Which are used for the therapy of rheumatism and benign prostate hyperplasia (BPH). In order to make full use of the plant resource in the northwest of China and the information source of foreign countries, we carried on a system research on the chemical constituents of U. Cannabina L.By using different chromatography techniques, 13 compounds were isolated from the 80% EtOH extract. Based on modern spectroscopic analysis technique and chemical methods, the structures of 10 compounds were elucidated as esculetin, scopoletin, p-coumaric acid, methyl P-coumarate, hentriantane, nonacosane, 3,5-dimeth--oxy-4-hydroxybenzaldehyde,Kaempfero1-7-0-rhamnoside, -sitosterols and daucosterol.This is the first report that compounds are in Urtica genus and all compounds except IX and X were first reported in Urtica cannabina L.Fatty acid from petroleum part was subjected to GC-MS after saponificated and methanolized. The derivative were composed of 62 components, 25 of which were identified, account for 74.04%. The content of palmitic acid was 23.23%, while stearic acid, eicosanoic acid, heneicosanoic acid, docosanic acid and tetracosanoic acid were more than 3% respectively.Organic acid was one of anti-inflammation active component in Urtica plant. General organic acid was extracted and methanolized to be analyzed by GC-MS. 60 peaks were inspected in NIST Dictionary, 27 peaks wereidentified, account for 71. 63%. The content of p-coumaric acid was 27. 86%, while cis-aconitic acid, methyl paraben, vanillic acid, 2,5-dihydroxy-benzoic acid, nonane dioic acid, citric acid were more than 3% respectively. Among them, p-coumaric acid, cis-aconitic acid, vanill ic acid, citric acid were well known antiinflammation active components. Fumaric acid, citric acid, succinic acid existed in Urtica Cannabina L. as well as in Urtica diocio.The severe consequence of hepatic fibrosis is cirrhosis liver . Preventing hepatic fibrosis is an important target to treat cirrhosis of liver. The preseription for benefited Qi to promote flow of blood was made up by Jian Wang et al. based on Chinese Traditional Medical Principle and clinical experience, which concludes Rheum palmatum L., Salvia miltinrrhiza Bge, Astragalus membranaceus (Fisch) Bge .var. mongholicus (Bge),Hsiao, Prunus PersicacL. Batsch, Polygonum multiflorum Thunb. It shows protective effects against rat Hepatic fibrosis in vivo. Lipophilic extracts show significant inhibiting effect at very low concentration.To further study the chemical constitutes of lipophilic active parts , we prepared Alc95% and Alc50% according to original literature. 20 compounds were isolated by using macroporous resin, silica gel, polyamide, Sephadex LH-20, Rp-18, preparative TLC and preparative HPLC chromato--graphy. The structures of them were elucidated by means of physical and chemical properties, modern spectroscopic analysis techniquesæ¡¿V, IR, EI/MS,ESI/MS,1H-NMR,13C-NMR,DEPT,HMQC,HMBC. They are nine anthraquinones: chrysophanol (95-1), physcion (95-2), emodin (95-5), aloemodin (95-7), rhein (50-1) , sennoside A or B(50-6) , -hydroxylemodin (95-10) , chrysophano1-8-0-D-glucoside (95-12), aloemodin-0-D-glucoside (95-13); three phenanthaquinones: tanshinone A (95-3), cryptotanshinone (95-6) , tanshinone I (95-4); one isoflavone: 7,3' -dih...
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