Ⅰ Isolation And Structure Identification Of Active Compounds From Soil Actionmycetes 1146 Ⅱ Quality Study On Medical Material Of Urtica Cannabina L.

I Isolation and Structure Identification of Active Compounds from SoilActionmycetes 1146Microorganisms are an important resource for biological compounds. The structural diversity and complexity of microbial secondary metabolites play an important role in exploitation for drugs development. The discovery of active metabolites is a meaningful work.In this paper we isolated active compounds from soil actinomycete No. 1146 collected in Yunnan Porvince for suppression on K562 cell growth. Firstly we compared nine different culture media and selected No.4 medium for fermentation in large volume and the fermentation broth was extracted with ethyl acetate and separated by silca gel column chromatography and RP HPLC. Structures of these compounds were further identified by physicochemical characteristics, MS and NMR.The main active compound isolated was identified to be actinomycin D. Other compounds were identified to be genistin, daidzein and phenylacetamide respectively.II Quality Study on Medical Material of Urtica cannabina L.Urtica L. has been widely used as folk medicine for a long history. Recent studies show that Urtica L extracts have anti-inflammatory, analgestic effects and inhibition of benigh prostatic hyperplasia(BPH). The main active components have been reported to be polysaccharides. In this paper we quantitively compared polysaccharide content in different species...