The Flavored Small Xianxiong Soup, Protective Effect On Vascular Endothelial Cells

Objective: To probing effects and theoretical clinical foundation.of add taste Xiao Xianxiong Tang on treating chest Bi by arranging ,analysising about the phlegm and the phlegm and stasis close mutual documents.Through observing add taste Xiao Xianxiong Tang contain medicine serum influencing on the cell model that oxidize lows density lipoprotein damage , discussing that add taste Xiao Xianxiong Tang treat chest Bi role and mechanism.Methods: Theoretical research: With document, collect and arrange , the method of analysis, demonstration and summary. Test research: Cell training is away from body experiment law. 1 Add taste Xiao Xianxiong Tang contain contain medicine serum the blood vessel for that oxidize lows density lipoprotein to guide leather cell hyperplasia and wither to die influence experiment ( MTT law and PI dyeing flow out type detection law ). 2 Add taste Xiao Xianxiong Tang contain medicine serum the blood vessel for that oxidize lows density lipoprotein to guide leather cell secrete active material NO/ET - 1 influences experiment (the enzyme reduction law of nitric acid with put the law of exempting ). 3 Add taste Xiao Xianxiong Tang contain medicine serum the blood vessel for that oxidize lows density lipoprotein to guide ICAM-1 that leather cell expresses and the quantitative and mRNA horizontal influence of egg white experiment ( immunity group RT, Westenblotting and the law of melting — PCR law ). 4 Add taste Xiao Xianxiong Tang contain medicine serum the blood vessel for that oxidize lows density lipoprotein to guide the influence of the cremation of the nuclear transcribe factor of leather cell ( NF - kB ) experiment ( immunity group the law of melting ).Results: there are many factor in the forming of chest Bi though theoretical research is considered , produce is the result of factor compound role, but phlegm turbid livelihood, retardance pulse road is crucial as becoming sick. Phlegm is turbid to produce with outside sense, internal injury and feelings will various factor influence the function imbalance of internal organs is related closely. Phlegm turbid easy heat with Yu close mutual, form phlegm heat close mutual to deliver stasis pathology change, and directly affect chest Bi occur development. Settle thermalization phlegm alive blood to treat chest Bi important rule. Add taste Xiao Xianxiong Tang to have treatment chest Bi phlegm and stasis the theoretical basic and clinical practice verification that closed mutual. Experiment research result shows: 1 Add taste Xiao Xianxiong Tang contain medicine serum can promote the leathercell in blood vessel hyperplasia , it is deceased to restrain it to wither, and with dosage have certain relation. 2 Add taste Xiao Xianxiong Tang contain medicine serum can Jie fight the leather cell in blood vessel secrete active material NO/ET - 1 lose weighing apparatus ( pass through tune NO content with ET - 1 last litres ). 3 Add taste Xiao Xianxiong Tang contain medicine serum can reduce leather cell ICAM-1 in blood vessel express and for its protein express quantity and its mRNA express level have influence. 4 Add taste Xiao Xianxiong Tang contain medicine serum can still restrain leather cell NF-kB cremation, restrain its nucleus obviously express in blood vessel.Conclusion: The cure facter is that the phlegm and the phlegm and stasis close mutual ,though there are many facters in the form of chest Bi.Add taste Xiao XianxiongTang treatment chest Bi have solid theory of traditional Chinese medicine make guidance, also have early stage clinical face experiment foundation. Add taste Xiao Xianxiong Tang contain medicine serum can anti-oxidize low density lipoprotein on damaging the cultured vein endothelial cell, have on role on protecting endothelial cells ,blocking initiation of atherosclerosis., there is great theoretical and experiment supporting value for the usuage of the Add taste Xiao Xianxiong Tang on coronary heart disease.