| Lung cancer is the most common cancer in both female and male persons worldwide, with estimated 1,352,132 new cases diagnosed and 1,178,918 deaths in 2002 (WHO 2002). The incidence is higher in developed and industrialized country than in developing country. Lung cancer represents 30.9% of all incident cancer in the world in men and 12.6% in women. Over the past decade, the incidence of Lung cancer in China has increased quickly. The incidence of Lung cancer in China was 269650 in man and 126718 in woman in 2002 (WHO 2002). Meta-analysis reveal that there was considerable pessimism about the role of chemotherapy in the treatment of non-small cell lung cancer. There is evidence for a modest increase in survival (6% at 1 year and 3% at 2 years) in patients with better performance status given higher dose radiotherapy.Kanglaite Injection (KLT) is a novel type dysphasic anticancer injection prepared by extracting its efficacious anticancer component from a Chinese crude drug/coin seed (Semen Coicis) for intravenous as well as intra-arterial injection. KLT not only has the obviously anti-lung metastasis which has the similar inhibitory rate with Cyclophosphamide, but also can strengthens body's immune functions. Experiences obtained from clinical application have demonstrated that KLT is significantly raise patient's immune functions and improve his quality of life. Our previous investigation found some differently expressed genes about immune such as IgE-BF, Titin, Lcp1 et al.In this study, we have customed a mouse 50-mer oligonucleotide microarray to assess the gene expression profile of LA795 mouse with lung adenocarcinoma by Kanglaite Injection versus negative control, and normal lung also be investigated. A total of 150 items of Oligo gene chipincluding 93 items of differential expression gene in our prophase experiments, 37 items of cancer immune associated genes searched from literature and 20 items of house-keeping gene made to our order by Beijing Boao Bio-chip Company Ltd. Were selected. The mouse lung tissues of normal control group and tumor control group were mixed up. In KLT group, the lung tissues of 3 mice were randomly selected to be labeled as KLT1, KLT2 and KLT3 for extracting mRNA separately and for having Oligo chip made to order.The difference of the same differential expression genes, filtered from 3 KLT samples chips was larger than 1.5-fold, and we got 10 items of tendency-identical genes, including 8 items of the gene being up-regulated expression, and 2 items exhibiting down-regulation. In comparison with normal control group, the tendency-identical genes had 6 items, among which the expression of tumor-inhibitory gene cyclin B2 in stage G2/M, and blocking of the cellular G2/M-phase to reduce the abundant expression of cyclin B2 in stage G2/M, and blocking of the cellular G2/M to reduce the mitosis, the tumor cellular proliferation was thus suppressed. The accelerator of the mouse Cyclin B2 is regulated by cell-cycle dependent cyclin (CDC), and the effect of CDC2 in promoting cell transformation is definitely associated with Cyclin B2. Therefore, KLT induced upregulation of Cyclin B2 expression may contribute to its effect in inhibiting the transformation of the tumor cells via checking CDC2 expression. Along with the development of tumor, the activity of alpha-1 protease inhibitor is suppressed. In the present study, after application of KLT, the alpha-1 expression was upregulated, showing that KLT may suppress the growth of tumor.Based on the therapy characteristic of the Traditional Chinese medicine, especially the conception of traditional Chinese Medicine synthesis treatment, we designed a clinic case analysis database using the...
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