Compound Ginkgo Dripping Pills Prevention Of Vascular Dementia And Its Mechanism Analysis

With aging aggravated, the incidence of dementia in senectitude shows the elevated trend year by year. The species of disease is thought to be focus not only in basic but also clinic medical science nowadays because of their high incidence, mutilation and mortality. Depending on its pathogenesis, dementia can be divided into Alzheimer's disease(AD), vascular dementia(VD) and mixed dementia. With the related research deepened gradually, all indications make clear that there exist many interactions between AD and VD in clinical situation, pathogenesis, etiology, pathology and epidemiology.VD is a kind of chronic brain syndrome induced by brain damage due to a series of agents related to cerebral vessels, mostly being accompanied with lasting and frequent cognition dysfunction. Epidemiology investigation shows that in western countries the incidence of dementia is 16%-29% among people elder than 60 in which the percentage of VD is 12%-37.2%. While in Asia and other developing countries, the incidence of VD is a little higher than that of AD. In Japan, VD holds 60%-70% in total dementia. Survey from 11 cities in China shows that the incidence of VD is 324/100,000 among people elder than 60, which is 60%-70% of total dementia. Owing to so high incidence of VD, the quality of life is affected seriously, which further results in great social and family burden. Therefore, it is imperative that developing effective drug for VD prevention be aimed at.Compound of ginkgo biloba extract dropping pill(CGDP) is mainly composed of ginkgo biloba extract and ginsenoside and used to prevent and treat VD and cerebral functional decline. The prescription with the advantage of slighter side effects, lower dose and more exact components is made up under the instruction of Chinese medicine theory and takes both Chinese and western medicine characteristic into account. Thus, developing CGDP for newer and more effective drug for VD prevention is not only social need but also embody of modern Chinese medicine research.In the dissertation, the effects of CGDP were investigated in vivo and in vitro respectively on tissue, cellular and molecular levels depending on the current researches on major components in CGDP, VD and cerebral functional decline. The methods in the research included praxiology, immunohistochemistry and biochemistry. Radioimmunology, enzyme-linked immunosorbent assay(ELISA) and molecular biology techniques were used to discuss its further mechanisms.1 Effects of CGDP on ameliorating cerebral functional decline in imitated dementia model murine and its primary mechanismsLearning and memory ability decline is a major clinical situation in dementia in senectitude. In this experiment, multi-infarction dementia(MID) rats model caused by thromboembolus of same kind of rats injected into unilateral internal carotid artery and cerebral functional decline mice model induced by D-galactose injected subcutaneously were employed respectively to investigate the effects of CGDP on learning and memory ability, biochemistry and pathology in model murine.1.1 Protective effects of CGDP on MID model ratsMID rats model was created by thromboembolus of same kind of rats injected into unilateral internal carotid artery. Morris water maze test, spectrophotography, HE staining, and ABC immunohistochemistry were employed respectively to detect the difference of learning and memory ability, lactic acid(LA) content, acetylcholinesterase(AChE) activity, cerebral infarction area and the express of brain-derived nerve growth factor(BDNF), glial fibriliary acidic protein(GFAP) in all groups of rats. The results showed that CGDP 10, 20, 40 mg/kg could improve learning and memory ability in model rats to different degrees after administration for 41 d continuously, which was proven by shortened escaping latency and time for first passing through the platform in Morris water maze test. After 46 d, CGDP could sustain cholinergic nerve system function by strengthening AChE activity and relieve anoxia of brain via reducing LA content. HE staining manifested that...