Scutellaria, Bupleurum And Its Compatibility Extracts Of Cvb <sub> 3m </ Sub> Proliferation And Myocardial Apoptosis Intervention Study

Objeetive1. To study the therapeutic effects of the skullcap extract, the Bupleurum extract and their compatibility extract on Coxsackievixus B₃a. (CVB₃m) in vitro, and to explore the antiviral mechanism, and to observe the influence on cell infected by CVB₃m.2. To observe the therapeutic effects of the skullcap extract, the Bupleurum extract and their compatibility extract on the 3alb/c mice myocarditis infected by CVB₃ and to exnlore the mechanism of the effect . Methods1. We used microcell culture method to observe the effect of the skullcap extract, the Bupleurum extract and their compatibility extract on antivirus and protecting cell. We detected the restrained indexes of the three kinds of drugs on CVB-3m and the cell protection rate of the three kinds of drugs when the cell was infected by CVB₃M2. Balb/c mice were inoculated intraperitoneally with CVB₃m and batch sacrificed late. On the base of the models , following experiment were carried.(1)The CVB₃m-RNA were detected by reverse transcription - polymerase chain reaction (RT-PCR), the heart histopathologic (macro- and ultramicro) were observed by the light microscope and electron microscope.(2)Mice were randomly divided into 5 groups, including the model group, the control group, the skullcap extract group, the Bupleurum extract group and the compatibility extract group. We cured the myocarditis of mice by intragastric administration for 14 days, and observed the body of weigh of mice, appetite, thesymptom and the death of mice. We detected the tite of CVB₃m, in heart, rate between the heart mass and weigh of the body and macro-histopathologic and ultrandcro changes of hearts.(3) The CVB₃m-RNA and IFN mRNA in heart were examed by RT-PCR method to explore the effect of antivirus and induction of IFN by the three kinds of drugs.(4)The apoptosis in heart were examed by TDT/Dutp Nick End labeling (TUNBL), and the apoptosis related protein and gene expression of Bc1-2 /Bax were detected by RT-PCR method and immunohistochemistry method .Result Experiments in vitro.1. The skullcap extract can directly kill CVB₃m. in vitro when his density is 1. 563 mg/ml, but the Bupleurum extract and their compatibility extract hadn' t this effect. The three kinds of drugs can protect cell infected by CVB3.. The effect of the Bupleurum group and the compatibility group is better than the skullcap groups when the drugs were dministered in advance. The effect of the skullcap group and the compatibility group were better than the Bupleurum group when the drugs were dministered after infection.2. The restrained indexes of the crude extracting skullcap was more than 2 when his density is TD₀; The restrained indexes of the fine extracting skullcap was less than 2 when his density is TD₀, 1/2TD_O and 1/4TD₀. The two kinds of drugs can protecte cell infected by CVB₃m , and the effect of the crude extracting skullcap was better than the fine extracting skullcap. Experiments on animal.1. The positive percent of CVB₃m-BNA in heart is 100% during 3th ¹4th day after infected. The heart histopathologic change were inflammatory cell infiltration, dropsy and necrosis of heart cell.2. The three kinds of drugs can increcse the body weight of myocarditis mice, appetite and the rate of survival; In increasing the rate of survival and the body weight, the difference of the three groups is not significant . The three kinds of drugs can inhibit replication of CVB₃m. in heart. The viral titer in heart of the skullcap group were lower than the model group in the3th, 5th, 7th and 10th day: The viral titer in heart of the Bupleurnm. group were lower than the model group in the 3th, 5th day: The viral titer in heart of the compatibility group were lower than the model group in the 5th, 7th day: The macro-, histopathologic and ultramicro changes of mice hearts of the three drugs were better than the model group too, but the effect of the Bupleurum group and the compatibility group was better than the skullcap group.3. The content of CVB₃m-RNA in the hearts of the three drugs...