Feasibility Study. Body Fibroblast Cells Were Injected Transplant

For all the plastic surgeon, it is an highly challenge to fill the soft tissue on the face, which focus on the injectable soft tissue filler. Since Wegener firstly reported to use the liquid silicon gel inl957, it has gone through countless difficulties and obstacles to exploit the injectable soft tissue filler. Even those, it had not made the satisfactory and had not been solved in the day.The injectable soft-tissue mainly include the manufacture, the organism filler and the active organism filler. The manufacture, such as the liquid silicon gel, the silicon particle, Artecoll and Arteplast, could cause the histology reaction to undegratable manufacture, to augment the soft-tissue. There were macrophage migration, fibroblast penetration and new collagen deposition. The organism filler, for example, Zyplast, Fibral, Hyaluronic Acid Derivatives, could not persist in the implant sites, and had been resorbed by the body, so the persistence of correction was not gratifying. Besides, adverse reaction to these above mentioned substance can occur on an allergic and nonallergic basis. These transient or durable reactions include bruising, swelling, ulcer, the local inflammatory responses, as well as the systemic immune disease. The plastic surgeon and patient are looking forward to the good biocompatibility materials to emerge.The active organism soft-tissue augmention, which should not only take part in the normal physiological metabolism, but also play role in excreting many growth factors or other functional protein, had to be the living cell transplantation. In the present, there are fat cell transplantation, fibroblast transplantation and cartilage cell transplantation in the plastic surgery field. Since 1995, in the University of Medicine and Dentistry of New Jersey, Department of Plastic Surgery, Hackensack University Medical Center, NJ, autologous cultured fibroblast(Isolagen) have been utilized successfully as a living, dynamic protein repair system for dermal and subcutaneous deficiencies. The autologous fibroblast culture process was started with a 3-mm retroauricular punch biopsy, which were expanded by proprietary tissue culture techniques. Cells of early passages(4, 5, 6) were used to treat rhytids, depressed scars, subcutaneous atrophy, ache irregularities, and laser wounds. A total of 4800injections were given on 1450 cases. Long-term follow-up ranged from 36 to 48months. A subjective patient satisfaction survey showed 92% of the patients were satisfied with grade of correction. A long-term follow-up survey revealed continuing improvement beyond the initial correction in 70% of patients. The following study showed that cultured fibroblast were functionally active and produced large quantities of type I collagen. In vitro studies of scar formation potency of injectable fibroblasts showed that these cells possessed normal collagen gel contraction capacity. In vivo experiments showed that that cultured fibroblasts exhibited no oncogenic properties and induced no tumors in nude mice. The study is designed to discuss the possibility of the autologous fibroblast to augment the mini-deficiencies. The whole study include 5...