Hainan Wild Fan Of Flowers And Grasses And The Chemical Constituents And Mass Spectrometry - Mass Spectrometry

Two Chinese medicinal herbs, the roots of Sarcococca vagans and the seeds of Allium tuberosum were subjected to phytochemical studies , respectively. 27 compounds were isolated, among them 9 were new constituents . Their structures were established by means of various modern spectroscopic techniques and chemical derivatization. In addition, tandem mass spectrometry (MS/MS) was applied to study the constituents in the total alkaloids from the roots of S. vagans and 10 trace constituents were structurally elucidated by the CED MS/MS of their [M+H]⁺ ions and 8 of them were new steroidal alkaloids.Phytochemical examination of the roots of S. vagans led to the isolation of seven new steroidal alkaloids , sarcovagine A^D (1⁴), sarcovagenine A, B, D (7⁹), together with the known axillarine F (5), pachysamine A (6) and H (10). The structures of the new compounds were determined as 20α-dimethylamino-2β, 4β-dihydroxy-3β-tigloylamino-5α-pregnane (1), 20α-dimethylamino-2α-hydroxy-3β - tigloylamino -4β-acetoxyl -5α-pregnane (2), 20α-dimethylamino-3β-tigloyl amino - 4β - acetoxyl -5α- pregnane(3), 20α-dimethylamino-3-tigloylamino-5α-pregna-2-en-4-one (4), 20a-dimethylamino-2β, 4β-dihydroxy-3β-tigloylamino-5α-pregna-16-ene (7), 20α-dimethylamino-2α-hydroxy-3β-tigloylamino-4β-aetoxyl -5α-pregna-16-ene (8), 20α-dimethylamino-3-tigloylamino-5α-pregna-2,16-dien-4-one (9), respectively. An unusual phenomenon that the carbon and proton signals from C₁₇-side chain were not discernible in the NMR spectra of compounds 1⁵ was discussed.The ESI-MS analysis of the total alkaloids from the roots of S. vagans demonstrated the presence of several trace constituents in the total alkaloids in addition to the isolated compounds. Eight new steroidal alkaloids, named...