Chemical Constituents Of Two Plants From The Genus Camellia Section Thea

This thesis elaborated the chemical constituents of two genus camellia sectionThea plants: Fangcheng tea (C. fengchengensis) and chuannan queshe tea (C. sinensisvar. sinensis). Eleven compounds including three new flavan-3-ol dimers wereisolated from the leaves of C. fengchengensis, by using various columnchromatography and other techniques. These compounds referred to ten flavan-3-olsand one simple phenolic compound. Their structures were elucidated by the detailedspectroscopic analysis, including1D,2D NMR, UV, and MS techniques. The volatileconstituents of chuannan queshe tea (C. sinensis var. sinensis) were obtained byhydrodistillation and analyzed for the first time by GC-MS. Meanwhile, the contentsof eleven phenolic compounds and caffeine were quantitatively analyzed by HPLCmethod. In addition, the chemical and bioactivity researches on the section Thea (L.)Dyer were reviewed.Chapter1. The chemical constituents of fangcheng tea.C. fengchengensis Liang et Zhong, belonging to the genus Camellia sect. Thea.,is an endemic species distributed in Fangcheng city, Guangxi province, China. Knownas a wild tea tree, its leaves have been used commonly for producing black teabeverage by the local people of its growing area. In this thesis, it’s the first time toinvestigation on the chemical constituents of Fangcheng tea guided by HPLC, whichled to the identification of eleven compounds, including three new flavan-3-oldimmers from C. fengchengensis Liang et Zhong. Compared with C. sinensis var.assamica, the chemical constituents of C. fengchengensis Liang et Zhong were totallydifferent and feature in flavan-3-ol dimmers. The study provide the chemicalsupporting for C. fengchengensis used as tea by local people in China.Chapter2. The chemical constituents of Chuannan Queshe tea. Chuannan Queshe tea is a kind of green tea made from the buds, and the first orsecond leaves of C. sinensis var. sinensis (Theaceae) growing in the Jinfeng mountain,located in the northern areas of sichuan province, China. The volatile constituentswere obtained by hydrodistillation and analyzed for the first time by GC-MS. Sixtycompounds, including twenty aldehydes, ketones and acids, thirteen alcohols, eightesters, ten aliphatic hydrocarbons, and nine other compounds were identified.Meanwhile, the contents of gallic acid, theogallin, gallocatechin, epigallocatechin,catechin, epicatechin, epigallocatechin-3-O-gallate, gallocatechin-3-gallate,epicatechin-3-gallate, strictinin,1,2-di-O-galloyl-4,6-HHDP-β-D-glucose, andcaffeine were quantitatively analyzed by HPLC method. The results showed that thecontent of main catechins in Chuannan Queshe tea distribution within the range of theteas, while below the average of the samples being investigated, especially caffeinecontent below rated minimum tea content of samples.Chapter3. The advance of chemical and pharmacological researches of theplants from the genus Camellia section Thea (L.) Dyer.