Involved In The Treatment Of Ischemic Cerebrovascular Disease Basis Of Experimental And Clinical Research

Background and purpose:Stroke is the most common and frequently life-threatening neurological disease and remains the major cause of disability among adults. Although we have known more and more about the mechanism of this disease, an effective treatment has not yet been established. Development of new therapeutic methods is warranted. We investigated the disease through experimental and clinical studies, expecting helpful gains in the field.一, Basic experiments[Purpose]To investigate HGF gene expression and biological effect after gene transfection into penumbra tissue in rat cerebral ischemic model.[Materials and Methods]1, A stable and reversible focal ischemia model with unilateral middle cerebral artery occlusion was established by inserting a nylon surgical thread through the internal carotid artery in rat, and was evaluated by CT perfusion imaging and TTC staining.2,Human hepatocyte growth factor gene was cloned from the human fetal liver cDNA library, and the expression vector (pIRES2-EGFP-HGF) was constructed by inserting the hHGF cDNA into an eukaryotic expression plasmid drived by CMV promoter/enhancer.3,The recombinant plasmid was transfected into rat's primary neurocytes with liposome. The expression of HGF protein was observed by fluorescence microscopy , immunohistochemistry and ELISA. Control groups were set. 4, Cationic liposome/plasmid DNA complexes(lipoplexes) were injected directly into the penumbra tisssue. Brains of treated and control animals were analyzed 7 days later.