Experimental Study Of The Monkey Brain Selective Deep Hypothermia Cerebral Blood Flow Recovery Protection Mechanisms

The metabolism of brain relies on the continual oxygen. The neuro can only tolerate4-5 minute hypoxia-ischemia at normal temperature and possible damage would beexist if beyond this confine. At neurosurgical clinic, blood flow occlusion partially orwhole is indispensable and essential which determine the curative effect of such diseaseas intracranial complex haemal disease, meningioma with abundant blood,reconstruction between intracranial and extracranial blood vessal, et al. At present,whole body deep hypothermic circulatory arrest(DHCA) was used but there are manycompliment which limit its broad use of DHCA such as the interval of blood flowocclusion less than 60 min, the deterioration of hematology, cardiac arrhythmias ofcardiovascular system and acute respiratory failure of respiration system. However,cerebral selective deep hypothermia combined with antegrade cerebral perfusion can notonly avoid these side effects effectively but also extend the limitation of blood flowocclusion by more depressed metabolism, which was identified by experiment of dog.Otherwise, selective DHCA combined with antegrade cerebral perfusion (ACP) canprovide efficient perfusion, sufficient electrolyte and take away substance produced.The cerebral of macaca mulatta is similar to the human beings in different sides, so it isthe necessary that we do this experiment in macaca mulatta. We established the mode ofmacaca mulatta of resuscitation after selective cerebral ultra-deep hypothermia andblood flow occlusion three years ago, on the base of this, We improve the ways ofpreparing the model of macaca mulatta. in order to explore the mechanism which reducethe compliment and is resistant to hypoxia-ischemia, we do something as follow: 1. tostudy preparation of macaca mulatta model of resuscitation after selective cerebralultra-deep hypothermia blood flow occlusion and to identify the model by pathologyand ultra-structural pathology. 2. To investigate magnetic resonance imagine(MRI)diffusion-weighed imagine (DWI) and the appearance and feature of 1H/protionmagnetic resonance spectroscopy(~1H-MRS) of macaca mulatta of resuscitation aftercerebral selective ultra-profound hypothermic and blood flow occlusion, To exploreffects of DWI and cerebral biochemical metabolism on resuscitation of cerebral...