Studies On The Chemical Constituents Of Anemone Altaica, Hemistepta Lyrata And Euphorbia Latifolia

In this doctoral dissertation, the phytochemical and pharmacologicalstudies were carried out on the medicinal plants of Anemone altaica Fisch. ex C. A.May, Hemistepta lyrata Bunge and Euphorbia latifolia Meyer ex Ledeb.. Meanwhile,the advances in the recent research of chemical constituents and pharmacologicalactivities of the genus of Anemone and Euphorbia were reviewed.Ten compounds (A1-A20) were isolated from the ethanol extract of the roots ofAnemone altaica Fisch. ex C. A. May by using the modem chromatography techniques.And on the basis of the spectroscopic analysis and chemical methods, their structureswere determined as follows: 4-(9H-β-carbolin-1-yl)-4-oxo-butyric acid (A1),carboxymethyl isoferulate (A2), isoferulic acid (A3), cirsiumaldehyde (A4),5-hydroxy-4-oxo-pentanoic acid (A5), triacontane (A6), palmic acid (AT),β-sitosterol(A8), daucosterol (A9), succinic acid (A10), cinnamic acid (A11), (2S, 3S, 4R,8E)-2N-[(2'R)-2'-hydroxy tetracosanoyl]-1, 3, 4-trihydroxy-8-octa decene (A12),protocatechuic aldehyde (A13), cimigenol-3-O-β-D-xylopyranoside (A14),cimigenol-3-O-β-D-xylopyrano(1→3)-β-D-xylopyranoside (A15), aralia cerebroside(A16), caffeic acid (A17), adenosine (A18), uridine (A19), methylβ-D-glucopyranoside (A20). Of the twenty compounds, A1 and A2 were new compounds, A3-A4 andA11-A20 were isolated from this genus for the first time. The pharmacologicalactivities of the compounds A1-A5 were tested on three models of Caspase-3, FactorⅩ, Thrombin. The .results showed that none showed strong inhibition activity.Discussion on the puzzlement of utilization of "jiujiechangpu" and "shichangpu" wasmade, providing reference on the reasonable administration. Twenty-nine compounds were obtained and identified from Hemistepta lyrata, ofwhich two were new compounds as follows: 8-carboxymethyl-p-hydroxycinnamicacid ethyl ester (L12), 8-carboxymethyl-p-hydroxycinnamic acid methyl ester (L13).Seventeen compounds were isolated from this genus for the first time as follows:hispidulin (L6), kaempferol (L8), salicylic acid (L9), protocatechuic acid (L10),8-carboxymethyl-p-hydroxycinnamic acid (L11), uracil (L14), caffeic acid (L15),succinic acid (L16), tracheloside (L17), acacetin-7-O-β-D-glucoside (L19),apigenin-7-O-β-D-pyranglycuronate methyl ester (L20), apigenin-7-O-β-D-pyranglycuronate ethyl ester (L21), 3-O-p-coumaroylquinic acid (L25), syringin(L26), salicin (L27), allantoin (L28), chlorogenic acid (L29). Other ten knowncompounds were identified as acacetin (L1),α-amyrin (L2), taraxasteryl acetate (L3),taraxasterol (L4),β-sitosterol (L5), apigenin (LT), daucossterol (E18),kaempferol-3-O-β-D-glucoside (L22), acacetin-7-O-β-D-rutinoside (L23),apigenin-7-O-β-D-rutinoside (L24).Eighteen compounds were isolated from Euphorbia latifolia, of which one wasnew: latifolin (E18). Seventeen were isolated for the first time, identified as lacceroicacid (E01), taraxasterol (E02),α-spinasterol (E03),β-sitosterol (E04), lupeol (E05),betulinic acid (E06), oleanolic acid (E07), betulin (E08), kaempferol (E09), luteolin(E10), quercetin (E11), daucosterol (E12), kaempferol-3-O-β-D-glucoside (E13),quercetin-3-O-β-D-glucoside (E14), 3, 4-dihydroxybenzoic acid (E15), gallic acid(E16), sucrose (E17).