The Animal Experiment Study Of Probiotics CL22 Therapy On The Chronic Gastritis Model In BALB/c Mice

It display that more than 50%people all over the world have infected Hp from the dates of epidemiology.The morbidity of Hp relation diseases like chronic gastritis,peptic ulcer,gastric carcinoma, mucosa correlation lymphoma is very high.it is a common disease in the digestive system.If we treat the Hp infection as pathogenic bacteria,the total amount of antibiotics each which used to eradicate Hp infection of all patients will be the most in all the pathogenic bacterium.Humen would suffer from the increase of drug resistant strain and adverse reaction of the antibiotics.At present therapy method of eradicate Hp is combination the two different antibiotics with protonpump inhibitor,which is effective,but with the increasing of drug resistance strain,the reinfection is hard to be resolved.On the other hand,people are resreaching and developing vaccine to prevent Hp infection,but we could not gain long-term immunoprotection even though the stomach itself infection Hp so it is very hard to develop a kind of effective and satisfactory vaccine.At present some articles reported that probiotics were effective to treat Hp infection.At the same time,we found from animal experiments that Hp only infected some animal species.Then,some people come up with the viewpoint that Hp should be treated as a conditioned pathogen.The idea evokes people's attention,because if it is right,We can prevent and therapy Hp infection through sustaining flora balance and correcting flora inbalance by drawing assistance from the reserach results of intestinal microecology.This viewpoint will take a new look on Hp therapy.The longterm antibiotics therapy method will be improved,we will only use them in short term finitely.In our study,we destoryed the normal flora in the stomach of Balb/c mouse,inoculated unacclimate Hp strain,then observed the stomach to find if it could plant or not.the study could help us achnowledge whether the flora of the stomach could influence Hp fixing planting in the stomach or not,which support the view that Hp is a conditioned pathogen.At last,we screened antagonistic from sinus ventriculi and carryed out treatment for animal models,the result would provid evidences to surport the new preventing view.Methods:1,Gastritis model induced by Hp in BALB/c Mice of clean Stomach1)Destroy the stomach flora though oral administered with antibiotics,acquire cleaned stage Balb/c mice of stomach flora inbalanced2)Administration by gavage inoculating 1×10~8CFU/mlHp to the mice stomach3)Control with the mice without destroying flora,execute the mice in 4 weeks and 8 weeks4)Stomach sinus ventriculi homogenate smear and cultivate Hp, fast urease test,paraffin section,Gimesa stain5)Histopathology analysis2,Separation method of native flora antagonism of Hp in the stomach1)Get sinus ventriculi mucosa through gastroscope into MRS medium anoxybiontic cultivating and separating mucosa flora2)In vitro solid microaerophilic culture,screening strain which can inhibit and kill Hp3)Growth character,Gram's stain,light microscope,electron microscope to check up morphology,biochemistry identification, metabolism product and Genetic character of the strain4)Stimulate the stomach circumstance to test resistence of the stain in gastric juice and bile acid.,3,Treatment method of gastritis induced by Hp iusing probiotics cl22 strain1)Establish the gastritis animal model of Hp infection,Balb/c mice model of cleaned stage2)Treat with cl22 for ten days,in contrast with PPI add two antibiotics,use the sodium chloride as corntrol3)Curative effect index detecting Hp in stomach and score of Hp in tissue damage vResults:1,The result of gastritis induced by Hp of cleaned stage model:1)Hp can succesfully induce the mice gastritis model of cleaned stage with gastric flora destroyed.2)The rate of Hp field plating in model group can achieve 100%.after 4weeks and 8weeks,The rate of Hp field plating in PPI+Hp group were 50%and 37.5%respectively,the rate of Hp field plating in PPI group were 37.5%and 25%respectively,The rate of control group is 0.1t has significant difference between the model group and other groups(p＜0.05).3)Histopathology analysis:The model group all had chronic gastritis after 4 weeks and 8 weeks;and the inflammatory degree became more serious with time passed;the grade of gastric mucosa inflammatory was much higest than other groups(p＜0.05).2,Separating result of native flora of Hpin the stomach1)The native probiotic Cl22 separated from human gastric mucosa was identified as Enterococcus faecalis,The propotion of GC was 36.9.2)The supernatant liquor of Cl22 strain cultivation can restrain remarkably the growth of Hp in vitro,and the germfree supematants can not restrain the growth of Hp.3)Our experiments proved that Cl22 has bile-tolerance and acid-tolerance effection.The main metabolic product of Cl22 is acetic acid.3,The result of treating gastritis induced by Hp with Cl221)Hp eradication rate:it was 70%in Cl22 group and 80%in PPI+antibiotics group,there were not obviously difference between them(p＞0.05).2)The gastric mucosa inflammate had a notable improvement after treated by CL22 or PPI mixed with antibiotics,their scores of gastritic mucosa damage had remarkable difference compared with the model control group(p＜0.05).but there were not difference between CL22 treatment group and PPI+antibiotics control group(p＞0.05).3)The score of Hp fix planting in the stomach mucose:there were not marked difference between CL22 treatment group and PPI+antibiotics control group(p＞0.05);The score of Hp colonization planting in control group had a significant difference compared with that of the model control group(p＜0.05).Conclusions:1)The gastritis mode in Balb/c mice challenged with Hp was successfully established in our study.It indicated that when flora in the mice gastric mucosa is normal,it can inhibit the Hp colonizing.It can establish gastritis model in Balb/c mice with Hp when the astric flora was destroyed with antibiotics.2)We successfully separated Cl22 enterococcus faecalis from human gastric mucosa which can inhibit and exterminate Hp.It is the native probiotics in the stomach.3)Cl22 could treatment the Balb/c mice gastritis induced by Hp effectively the curative effect is similar to using PPI and antibiotics.