Investigate The Effect And Mechanism Of The Treatment Of Melasma Cream Soup

Objective:Chloasma is an acquired pigmentation dermatosis.It has a complex mechanism.West medicine hasn't studied it completely and obviously.There is no satisfying treatment in chloasma now.Chinese medicine shows some superiority with its less side-effects and lasting curative effect.The aim of this study is to reveal the mechanisms of Quban decoction to cure chloasma.It can provide a theory basis for its clinical application on Chloasma.Methods:The inhibition of Quban decoction on tyrosinase was determined by the dopachrome method in vitro,and Quban decoction was investigated by anti-DOPA autoxidation activity,anti-ultraviolet catalysis activity,and promoting degradation of melanin.A375 human melanoma cell was employed in this study to investigate the effects of Quban decoction and Medicated serum of its on cellular proliferation, tyrosinase activity and melanin content.The model of chloasma in female mice was induced by musculal injection of progesterone for one month,the effects of Quban decoction on SOD,MDA and NO in skin and liver were detected,and skin melanocyte was observed.At the same time,samples were taken to store in the fixation liquid for HE and immunohistochemical performance.Results:The results showed that Quban decoction could inhibit the activity of tyrosinase,and at the same time it showed anti-DOPA autoxidation activity and anti-ultraviolet catalysis activity.Quban decoction and Medicated serum of its had the effects on inhibiting the proliferation,and decreasing tyrosinase activity and melanin content of A375 human melanoma cell in vitro.Quban decoction could increase the activity of SOD,while the contents of MDA were obviously decreased in skin and liver,and at the same time a decrease of melanin production in the mice model was observed.Conclusions:Based on the above experiments,we revealed the mechanisms of Quban decoction to cure chloasma,and its possible mechanisms may be related to inhibiting melanocyte roliferation,decreasing tyrosinase activity,and anti-oxidization, and so on.