The Spectral Information Studies Of Cervical Cancer Cells At Different Stages Of The Cell Cycle

Cell is the cornerstone of research in life sciences. All the key issues of life have to look for answers in cells. Cell division can be said to the most basic and most important process in origin of life. Cell cycle embodies all the physiological process in cell division, and it exists in higher creatures. A large number of studies have shown that abnormal regulation and control of cell cycle is the main cause of tumor, therefore the cell cycle theory gives directions to cancer prevention and clinical diagnosis and treatment of tumor.With the interdisciplinary of life sciences and optics, the study of cells'optical information has become a important area in life sciences, of course, the combination of optical technology and cell research will inevitably produce various problems, especially the cell cycle study using spectrum technology. On the whole, from the studies of domestic and foreign scholars, there are some questions about the spectrum study on cells which are in cell cycle.â‘ Single optical band. Almost all the research institutes and researchers did studies on the changes of cell cycle using infrared spectroscopy. They did not make full use of the existing spectrum technologies to give the cell cycle a comprehensive study.â‘¡Complex analysis methods. Biological cells have a wealth of information in the infrared band, because almost all the elements of biological cells have light absorption in the infrared band, which leads to the infrared spectrum aliasing. And the track of specific molecules in cells becomes very difficult.â‘¢No clear explanations and resolutions for some key issues in the spectrum study on cells which are in cell cycle, such as the access to samples, what kinds of synchronization level about the samples, methods of building cell cycle spectrum model, parameter extraction and so on.This article aimed at the existing problems, using ultraviolet and visible spectroscopy and fluorescence spectroscopy, extracted the spectrum characteristics of living tumor cells at different phases. The followings are our research work:â‘ Design and implement the synchronization processing plan about HeLa cells According to the principles of cell synchronization, the synchronization processing method about HeLa cells has been put forward, as well as the level of cell synchronization on the following experiments to obtain the HeLa cell samples which meet the need of the test requirement in the cell cycle Phase (G1 phase, S phase, G2 and M phase) .â‘¡Do experiments to obtain ultraviolet and visible (UV) absorption spectrum of HeLa cells in different cell cycle phases.Design the synchronization processing plan on ultraviolet and visible absorption spectrum about HeLa cells in different cell cycle phases. Then give ultraviolet and visible light absorption spectrum measurement on HeLa cell samples (190nm ~ 1100nm).The result shows that the spectrum of cell simple in different phases reflects the changing processes of chromophore and auxochrome with the changing HeLa cell cycle. Around 204nm and 260nm, the spectrum has two peaks. The former is caused by the aromatic amino acids, the latter by comprehensive absorption of protein and nucleic acid. These changes show that in the process of HeLa cells'growth ,UV absorption spectrum reflects the close relationship among the aromatic amino acids, purine and pyrimidine derivatives and HeLa cell growth process.â‘¢Do experiments to obtain the auto-fluorescence spectrum about HeLa cells in different cell cycle phasesDesign the experiment plan on auto-fluorescence spectrum of HeLa cells in different cell cycle phases. We got the autofluorescence spectrum of the samples exciting by 290nm light. The results indicated that the fluorescence spectrum has two peaks at 360nm and 680nm, the intensity of these two peaks changes in different samples. It shows that the materials producing fluorescence changes with the cell growth, resulting in the changes of fluorescence intensity. Its spectral curve reflects the trend of amino acid and porphyrin changing in HeLa cell cycle.â‘£Build HeLa cell spectral modelAccording to the ultraviolet and visible light absorption spectra measurements about HeLa cells, using the method of biological spetrum modeling, the linear regression model spectrum and multiple linear regression model have been put forward evaluated .These two models both can have a very good response to changes in the HeLa cell cycle in the process of UV absorption spectrum. There will not be further studies about auto-fluorescence spectrum due to the changes of characteristic peak indensity can not be described by linear regression model.Finally, the correct rate of regression model and multiple linear regression model achieved 98%, which determine the simple cell at which phase in cell cycle. It shows that the UV absorption spectrum model can describe the absorbance changes of characteristic peak in cell cycle of HeLa cells.