Prevention And Control Gk Rats. The Tetramethylpyrazine United Burnett Plymouth Concurrent Kidney Disease Experimental Study And Mechanism Discussed

Objective:To observe ligustrazine combined with benazepil tablets prevention and cure in expression level of intercellular adhesion molecule-1(ICAM-1),tumour necrosis factor-α(TNF-α) and osteopontin (OPN) in GK rats nephridial tissue tentatively and evalute the possible mechanisms of effects on diabetic neuropathy(DN) on earlier period. To probe into the connection between diabetic neuropathy and inflammatory to open out the possible mechanisms of DN for appraising and diagnosing new quideline on diabetic neuropathy on earlier period.Methods:Forty GK male rats were randomly divided into four groups: 10 every groups,respectively for model group,ligustrazine group, benazepil group and ligustrazine combined with benazepil group.Drench with NS 5ml·kg^-1·d^-1,benazepill.6mg·kg^-1·d^-1,ligustrazine 150mg·kg^-1·d^-1,ligustrazine combined with benazepil(150mg·kg^-1·d^-1+1.6mg·kg^-1·d^-1) to rats respectively,according to the group in 16 weeks.Puting big mouses into metabolism cages in the 8th week,the 12th week and the16th week,collecting urine to determine quantitation of Urine protein in 24 hours.Treat for 16 weeks,anaesthetize the big mouse by 3% pentobarbital,then cut open the belly of rats and pick its kidney, examine TNF-αand OPN content in renal organize by means of RT-PCR and ICAM-1 content by means of immunohistochemistry,and determine IL-6 level in blood serum and RBP level in emiction of every group.Result:Compared with other group,the blood glucose of model group rats increase obviously(P＜0.05),quantitation of 24h Urine protein rise obviously(P＜0.05),degrade the express level of TNF-α,ICAM-1 and OPN in renal organizes increase obviously(P＜0.05,或p＜0.01);blood Indexes such as IL-6 and RBP level in emiction are improved(P＜0.05,或p＜0.01). ligustrazine combined with benazepil tablets can protect the kidney for lowerring blood glucose,TG and TC,degrading TNF-α,ICAM-1 and OPN express level in renal organize,decreasing serum albumin,blaood urea mitrogen and increasing creatinine clearance,at the same time,reducing Urine protein and IL-6 levelConclusion:ligustrazine combined with benazepil can reduce great mouse blood glucose level of diabetic rats and improve nephric tubule pathological change,moreover;it can play its roles depend on resisting inflammation and anticoagulatory function.One of the possible mechanisms of DN is to degrade TNF-α,ICAM-1 and OPN express level in renal organize and reduce IL-6 level in blood serum.