Effect Of Trefoil Factor 1 On Induced Expression Of Intercellular Adhesion Molecule 1 And Vascular Cell Adhesion Molecule 1

Objective:To investigate TNF-αinduced expression of intercellular adhesion molecule 1(ICAM1),vascular cell adhesion moleculel(VCAM1) in human gastric epithelial cell GES-1,and to investigate the effect of trefoil factor 1 on the expression of ICAM1 and VCAM1 induced by TNF-α.Methods:Human gastric mucosal epithelial cell GES-1 were cultured in vitro.After incubated with different concentrations and durations of human recombinant TNF-α, RT-PCR and Western Blot were applied to measure the expression of ICAM1 and VCAM1 in mRNA level and protein level respectively,and the localization of NF-κB p65 protein was detected by immunofluorescence.The plasmid pIRES2-EGFP-TFF 1 was constructed,then was transient transfected intoGES-1 cells,the alteration of ICAM1,VGAM1 expression were assayed by Western blot.Results:1.TNF-αcould induce the expression of ICAM1 and VCAM1 in GES-1,as concentrations and induction times of TNF-αare rise,the expression of ICAM1 and VCAM1 increase correspondingly.2.In GES-1 cell,NF-κB p65 protein translocation from the cytoplasm to the nucleus afer TNF-αstimulated.3.Recombinant plasmid pIRES2-EGFP-TFF 1 transient transfected into GES-1 cell line,TFF1 expression was detected.4.TFF1 expression significantly induce TNF-α-induced ICAM1,VGAM1 expression in GES-1 cell(p<0.05)Conclusion:In the GES-1 cells,TFF1 overexpression attenuates significantly TNF-α-induced ICAM1,VCAM1.TFF1 may manifest a typical anti-inflammatory factor through the inhibition on adhesion molecules such as ICAM1 and VCAM1..