The Study Of Serum Creatine Kinase Abnormal Elevation Post-excercise And Their Relationship With ACE/CK-MM Gene Polymorphism

It was well known that high intensity exercise may lead to myalgia, increased creatine kinase(CK) and other symptoms of rhabdomyolysis,and even result in serious complications.However,some healthy people had CK markedly elevated after moderate or mild intensity exercise and their proportional was asymmetric,some scholars named these people,that CK obviously elevation post moderate or mild intensity exercise,as high responders(HR).After excluded the medical,fitness,training status,body fat content,and the lack of some enzymes,people considered that genetic differences made the phenomenon.In recent years our department also diagnosed several young soldiers as HR,which with fatigue,dark urinary and significantly increased serum CK after low-intensity training,some individuals recurrent episodes.A man and his son both were diagnosed as HR, so the HR related with gene.We thought two genes may related with HR after analyzed the gene of some HR.In order to find out distribution characteristic of HR in the Han population of China and to study the their relation with the ACE gene I/D polymorphism and restriction fragment length polymorphism(RFLP) in the CK-MM gene,we screened 201 healthy recruits to run 3000m and tested some the creatine kinase and other biochemical index.We defined the CK value of HR by centile of biochemical index,then detected and analyzed ACE/CKMM gene polymorphism by PCR and RFLP technique.The results showed that the CK value of HR was 577 IU/L,there are 20 subjects were the high responders.Between Han control and Caucasian control,the allele frequencies and genotype of ACE/CK-MM gene polymorphisms had significant difference(P＜0.05).the ACE gene frequency of HR in Han as follows:I=53%,D=48%,the D allele frequency was higher than Han control group,but no statistical significance(P=0.06); DD genotype(25%) also was significantly higher than that of control without statistical difference(P=0.06);Between the Caucasus group and Han HR, DD genotype and D allele were no statistical difference(P=0.10,P=0.29). CK-MM gene frequency of Han HR as follows:A=65%,G=35%.G allele frequency of HR was higher than that of Han control(P＜0.05);GG genotype also obviously higher than that of Han control(P＜0.05);The allele frequency and genotype of CK-MM in HR of Han population were no significant difference from that of Caucasian(P＞0.05),compared to the Han controls were significant difference with Caucasian(p＜0.05).Conclusion:1.To general population,about 10%of individuals prone to abnormal CK elevated post exercise,and the threshold of HR was 577IU/L in this study.2.ACE D allele related with HR.In this study,the ACE genotype distribution of the HR closed to the Caucasus control.3.CK-MM G gene had obviously correlated with HR.The CK-MM genotype distribution of the HR closed to the Caucasus control.4.The individual with GG/DD genotype was more vulnerable to CK abnormal increase post-exercise,D and G allele had a additive action to muscle injury after exercise.