Study On Chemical Therapeutic Basis And Compatibility Of Dachengqi Decoction

Dachangqi decoction,which derived from Treatise on FebrHe Disease,is a famous prescription that has been used to treat constipation over a very long period of time and proved quite a good effect.The extracted technology, chemical components and pharmacological activities and the compatibility of dosage change of Dachengqi decoction was study on this researchThe HPLC contents determination method of Anthraquinone Components (Aloe-emodin,Rhein,Emodin,Chrysophanol,Physcion),Flavonoids Components (Naringin,Hesperidin,Neohesperidin),Phenols Components(Honokiol, Magnolol) were establish,these method are simple,reliable,accurate.The traditional 'one - factor at a time' technique was used to identify the level and extent of all 3 factors of ethanol concentration,reflux time,and solvent ratio,then a central composite design-response surface methodology was used to optimize the extracting process for Dachengqi decoction,a two-order polynomial equation was fitted to the data and resultant model was used to predict the response in the optimal region.Analytical results indicated the optimum conditions were 60%ethanol,90 min for once reflux,20-fold solvent for extraction.It shows that the optimum process is simple and more convenient for extracting Anthraquinone,Flavonoids and Phenols components with an higher yields.A stable,reliable,uniform HPLC analytical method with good reproducibility was developed to study the changes of chemical ingredients between single herbs of Dahuang,Houpo,Zhishi,Mangxiao and the compound recipe of Dachengqi decoction.The results indicated that in the 49 peaks of Dachengqi decoction,there are 12 peaks contributed from Zhishi,12 peaks contributed from Houpo and 17 peaks contributed from Dahuang,but no new peaks produced.Comparing Dachengqi decoction with single herbs of Dahuang,Houpo and Zhishi,the areas of 24 peaks are increased while the areas of 8 peaks are decreased.The peaks areas of Naringin,Hesperidin,Neohesperidin, Honokiol and Magnolol didn't increase,but yields of Aloe-emodin,Rhein, Emodin,Chrysophanol were increased when four herbs decoct together,which also indicated that traditional decoction method of Dachengqi decoction was increase the Anthraquinone components of the medicine so as to enhance the effect to treat constipation.The quantitative contents of Aloe-emodin,Rhein,Emodin,Chrysophanol, Physcion,Naringin,Hesperidin,Neohesperidin,Honokiol and Magnolol were detested by HPLC in different compatibility of Dachengqi decoction as to study the active ingredient changed regularity of Chinese material medicine.The results show that Flavonoids and Phenols Components were hardly influence by different compatibility of Dachangqi decoction,but the contents of Anthraquinone components could become higher when Mangxiao and Dahuang were decocted later,and Houpo,Mangxiao,Zhisbi were compatible with Dahuang together can also improved the yields of Anthraquinone components,which proved that traditional decoction method of Dachengqi decoction was reasonable for increase the Anthraquinone components of the medicine as to enhance the effect to treat constipation.The method of charcoal powder propelling ratio was used to investigate the effects of Dachengqi decoction on the intestinal propulsive movements in mice,and observe the influence of Dachengqi decoction on the intestinal smooth muscle of the experimental rabbits.The results show that,compare the blank group,the compatibility of different groups all show the Correlation were significant at the 0.05 level or 0.01 for charcoal powder propelling ratio, tonicity and contraction of the rabbits' s isolated intestinal muscle.The groups of Dahuang combine with Mangxiao hava a good pharmacological activities indicate that Dahuang and Mangxiao have a sronger effect to treat constipation, as well as the group of tradictional method.The correlation between chemical components and pharmacological activities was carries on in order to study the therapeutic basis of Dachengqi decoction.49 peaks correspond to pharmacological activities were analysis by bivariate with the software of SPSS 16.0 for windows,there 11 peaks correlated to the pharmacological activity of Y₁(charcoal powder propelling ratio) with Correlation is significant at the 0.05 level or 1.01 level;3 peaks correlated to the pharmacological activity of Y₃(contracted frequency of isolated intestinal muscle) with Correlation is significant at the 0.05 level. The results indicated that Aloe-emodin,Rhein,Emodin,Chrysophanol are the therapeutic basis of Dachengqi decoction,and also explain the rationality and mechanism that these chemical components are used as to control the quality of herbs and recipe.Response surface methodology(RSM) involving Doehlert design was adopted to evaluate the yields of Anthraquinone components in Dachengqi decoction by change of dosage of Zhishi,Houpo and Mangxiao and shorten analysis time through a desirability function.The results of this research show that Anthraquinone components were got a high yields of predicable value of 0.85 when the dosage ratio of Dachengqi decoction were compatible as follows: Dahuang:Zhishi:Houpo:Mangxiao(1:4:2.31:2).The Doehlert design and desirability function,which allow a sequential response methodology,are a good methods,and,of cause,the mathematical model can be further popular and apply for the compatibility research of Chinese material medicine.This research was conduct under the theory of traditional Chinese medicine incorporated with science of Chinese materia medica,analytical chemistry, physics,statistics and technology of computer.In this research,the extracted technology were optimize by CCD,probe into the mechanism of compatibility,therapeutic basis of Dachengqi decoction,which provided a significative exploration for the modernization of compatibility of traditional Chinese medicine.