Empirical Study Of Acupunctunre Therapy Reverse Blood Vessel Endothelium Dysfunction In Insulin Resistance Model

Endothelium Dysfunction as the breakaway ring-joint of Artherosclerosis has an importance effect to happen and development of the disease.In this year we find that the insulin resistance will lead the Endothelium Dysfunction,and participative the happen of the Artherosclerosis.The IR was the metabolic disorder base of diverse disease which correlated with Metabolic syndrome include the glucose tolerance abnormal,type 2 diabetes mellitus,obesity,dyslipidemia and hypertension,it also was a very important reason of the end point incident of eadiovascular and cerebrovascular base diseades such as Atherosclerosis and coronary atherosclerotic heart disease.The overriding effect to the vascular is the chronic complication.Now the heat point of the base investigative of the vascular lesion was "the Dysfunction of Vascular Endothelium",it was said that the Dysfunction of Vascular Endothelium is the cectral link of the vascular lesion and also the origination link.Now protect the Vascular Endothelium,and reverse the damage of the Vascular Endothelium was the central link of prevention and cure vascular lesion,and it also the very difficult link of the Modern Medicine.Acupuncture as a naturopaths,because it uncomplicated,economical,convenient and efficacious,so it has the superiority for several thousand years to diagnose and treat in the tradition chinese medical science,furthermore many research confirm that acupuncture has a good effect that it can regulate the endocrine metabolic,the dominance had already gradually fadein in therapy many modern disease.Though the work about the acuouncture treat the IR carry out less in nowadays,but the curative effects of acupuncture to the DM, obesity and cerebral infrarcfion was already obtain the same trade or occupation internal and abroad in for a long time.The clinical and empirical study of the Predecessor had show that,the acupuncture had a certain effect of reverse the IR,the acupuncture can improvability the state of IR model and the dicbetic,can prevention and cure the chronic complication,decrease the develop rate of blood vessel chronic complication that owing to the state of hyperglycosemia,but the study above had not had a system study yet. The study was based in the grouding of the predecessor' study:use the high glucose, high fat,high salt diet to copy the IR model,and to combine the modern detect technology to investigate the change of the Vascular Endothelium function,and the effect of acupuncture to the change of the Vascular Endothelium function.This study was include three parts,the literature study:â‘ through study mass of literature about the IR,from the conception of IR,the Epidemiology of IR,the pathologic mechanism and prevention and cure of IR,the IR and the relationship disease,the relationship of IR and the dysfunction endothelium and the mechanisim of the happen and develop to expound the latest study of IR.â‘¡the realization and research of progression of the chinese medical science to IR,the research situation of chinese medicine to prevention and cure the IR,especially to analysis the domestic and aboard use the acupunture to prevention and cure the IR,to raise the coming trend of the study,consider that the method of acupuncture was a good mean to improve the IR,at the base of the study of predecessor we emphasized to study the of acupunture to the dysfunction of Vascular Endothelium in IR,inorser that to explored the machanisim of the acupuncture to the IR..Part one Literature researchThrough to massively about the insulin resistance's literature synthetic study,from the insulin resistance concept,the insulin resistance and the related disease related the influencing factor which as well as the insulin resistance,the insulin resistance occurred has the mechanism and the prevention and so on elaborated variously the insulin resistance's newest research development,and has carried on the analysis to the Chinese medicine prevention insulin resistance's research present situation,has carried on the narration specially to the acupunture therapy insulin resistance'sPart two Experimental studies2.1 MethodsUse the high glucose,high salt,the high fat diet copy the insulin resistance model big mouse,according to the blood sugar,the blood fats,the insulin sensitive index,the related cell factor(TXB₂,6-Keto-PGF1α,ET,NO) and so on judgments makes the mold success or not.To combine the immunohistology,pathology,Radioimmunodetection,etc,modern examination technology observation acupuncture union immunity group study resist the big mouse model blood sugar,the blood fats,the insulin sensitive index,are related the internal organs(liver,pancreatic gland) the morphology influence to the insulin,as well as responds the IR big mouse model blood vessel bast function the index of correlation:Blood rheology target,related cell factor(TXB₂,6-Keto-PGF1α,ET,NO),aorta VEGF expression influence and so on influence,key prominent acupuncture to IR big mouse model blood vessel bast function influence.2.2 Results2.2.1 acupuncture to the IR big mouse model general condition and body weight influenceIn the experiment process,the normal control group big mouse body weight quality increases gradually,the state of mind is good,responds keenly,the movement freely,the hair lies flat has the gloss,does not have 1 example death.The 5th week starts,the model 1 group,the model 2 groups,the acupuncture 1 group,the acupuncture 2 group of big mouse personality poikilothermia is suitable,the carriage is obese,is addicted to rests little moves, the big mouse superficial knowledge dishevelled does not have the gloss,the bowel movement quantity normal group are few,the color ash,the nature is soft,the body weight grows quickly,the abdomen is obese.The model 1 group of big mice which by Gao Zhi, Gao Tang,the high salty feed feeds have appeared gradually drink,the multi-food,the polyuria,and the energetic dispirited,the slow reaction,the movement is slow,the body weight growth is later period slow,in the experiment process dies 1.The model 2 groups after changing to the ordinary feed feeds(diet intervention),only the minority big mouse appeared drinks,multi-symptoms and so on food,polyuria,the body weight still had continues to grow,but the spirit compared model group of good,responded that the movement and so on has not seen obviously slow.In the experiment process has not seen the animal death.Continuously by Gao Yan,Gao Zhi,high sugar diet raising acupuncture 1 group of big mice(only acupuncture intervention),before acupuncture treatment the spirit, the response,the movement have the change for the better,in the experiment process die 1. The acupuncture 2 groups of big mice(diet and acupuncture intervention),treats 2 weeks later the acupuncture,the energetic dispirited,the slow reaction,before the movement slow and so on,the obvious change for the better,the body weight rate of rise obviously reduces, in the experiment process has not had the animal death.2.2.2 The influence of Acupuncture to IR big mouse model blood sugar,blood fats, insulin sensitive indexGives high glucose,high salt,the high fat diet 8 weeks later,each group of big mouse's blood sugar,the blood fats level obviously elevate,the insulin level obvious drop, compares with the blank control group,the difference is remarkable,p<0.01;The insulin sensitive index obviously reduces,compares with the blank control group,p<0.01.After diet intervention,the model 2 group of big mouse blood sugar level,the blood fats level and so on drop,the insulin sensitive index has the rise,compares with the model 1 group of big mice,the difference is remarkable,p<0.05;After the acupuncture treatment's acupuncture 1 group,the blood sugar,the blood fats level obviously drop,compares before the intervention and the model group,the difference is remarkable,p<0.05;After the acupuncture and the diet intervention treatment's acupuncture 2 groups,its blood sugar and the blood fats level drop obviously,the insulin sensitive index obviously rises again, compares before the intervention and the model group,the difference is remarkable,p<0.01; 1 group and the model 1 group compares with the acupuncture,the difference is remarkable,p＜0.05.2.2.3 The influence of Acupuncture to the IR big mouse model pancreatic gland,liver morphology'sThe model 1 group of big mouse pancreatic gland organization part has the yellow muddy phenomenon.The light microscope observation obvious island of langerhans peripheral has the massive lymphocyte infiltration,in the partial island of langerhans essence also has the inflammatory cell infiltration,the mesenchymal knot contracts the organization proliferation,is the membrane accumulation(Figure 3).Model 2 groups,after 4 week diet intervention,the big mouse pancreatic gland organization still had the yellow muddy phenomenon.The light microscope observation obvious island of langerhans peripheral has the obvious lymphocyte infiltration,in the partial island of langerhans essence also has the inflammatory cell infiltration,the mesenchymal knot contracts the organization proliferation,is the membrane accumulation.The overall pathological change degree compares the model 1 group to reduce relatively.The acupuncture 1 group of big mouse pancreatic gland organization obviously normal acinus and the island of langerhans structure,the partial pancreatic gland peripheral have the obvious inflammatory cell infiltration,in the partial island of langerhans essence also has the inflammatory cell infiltration;Has not seen the obvious knot to contract the organization proliferation,is the membrane accumulation phenomenon(Figure 4).the acupuncture 2 group of pancreatic gland organization obviously normal acinus and the island of langerhans structure,the part has the mild inflammatory cell infiltration.Has not seen the obvious knot to contract the organization proliferation,is the membrane accumulation phenomenon.Normal control group:The hepatic lobe structure is complete,the central vein comes,and the liver rope assumes the radiated arrangement to four weeks.Model 1 group:Obviously the proliferating liver cell fatty degeneration,becomes the host by the small vacuole fat,in the hepatic lobe has the phlogocyte to infiltrate(Figure 1).Model 2 groups:Obviously the proliferating liver cell fatty degeneration,becomes the host by the small vacuole fat,in the hepatic lobe has the phlogocyte to infiltrate(Figure 2).Acupuncture 1 group:Obviously disperses in the liver cell fatty degeneration,becomes the host by the small vacuole fat,in the hepatic lobe the little phlogocyte infiltration(Figure 2).Acupuncture 2 groups:The hepatic lobe structure is complete,the central vein comes,the liver rope assumes the radiated arrangement to four weeks,has not seen the obvious liver cell fatty degeneration, the hepatic lobe non-phlogocyte infiltration(Figure 2).2.2.4 Acupuncture to IR big mouse model blood rheology influenceThis experiment demonstrated:The IR big mouse model blood assumes Gao Niangao to congeal the condition:The blood mounts the stagnation markup,the blood corpuscle accumulation enhancement.After acupuncture and diet intervention treatment,can improve the IR model effectively the blood rheology target.Compares with the blank group,the model 1 group and the model 2 groups of high shears rate,the low shear rate,the blood plasma specific viscosity,the red blood cell press the product,the red blood cell electrophoresis time,the red blood cell accumulation index and so on obviously to be higher than the blank group(p＜0.01).Compares with the model 1 group,the acupuncture 1 group of high shears rate,the red blood cell press the product,the red blood cell electrophoresis time,the red blood cell accumulation index obviously to drop(19＜0.01 or p＜0.05),the acupuncture 2 groups of high shears rate,the low shear rate,the blood plasma specific viscosity,the red blood cell press the product,the red blood cell electrophoresis time,the red blood cell accumulation index obviously to drop(p＜0.01).Compares with the model 2 groups,the acupuncture 2 groups of high shears rate,the low shear rate,the blood plasma specific viscosity,the red blood cell press the product,the red blood cell electrophoresis time,the red blood cell accumulation index obviously to drop(p＜0.01).2.2.5 acupuncture to IR big mouse model SOD,MDA,GSH-Px influenceThe experimental result showed:The IR big mouse blood plasma oxidation - oxidation resistance system is unbalanced,the oxygen free radical product MDA level obviously rises, oxidation resistance levels and so on material SOD,GSH-Px obviously drop.After acupuncture and(or) diet intervention the MDA level drops,levels and so on SOD, GSH-Px have the rise,is best after the acupuncture and the diet intervention's acupuncture 2 group of big mouse effect,compares with the model 1 group,the difference is remarkable, 1 group and the model 2 groups compares with the acupuncture,the difference is remarkable.The prompt acupuncture possibly through adjusts IR model big mouse's oxidized - oxidation resistance system's balanced,plays the reversal insulin resistance the role.2.2.7 acupuncture to IR big mouse model aorta VEGF expression influenceThe model 1 group of big mouse aorta slice obvious yellowish brown color masculine gender expresses the signal,is mainly located in the cytoplasm and the extracellular is mesenchymal;Model 2 groups,the acupuncture 1 group of obvious yellowish brown color masculine genders express,but does not have the intensity which the model 1 group expresses;The acupuncture 2 groups may the becoming fewer scope express,but the normal control group expresses few.3 Conclusionsâ‘ High glucose,high salt,the high fat diet may succeed duplicate the insulin to resist the big mouse model,this model can display the essential feature which the insulin resistance should have:Hyperglycemia,high blood fats,high insulin blood sickness,centripetalism obese and so on.Confirmed after this research that this model big mouse blood vessel bast function has obviously suffers injury the performance,builds the good foundation for this research's smooth development.â‘¡The acupuncture intervention may obviously improve the IR model insulin resistance:Reduces the IR big mouse blood fats,the blood sugar,the improvement insulin resistance,with the model group contrast,the difference is remarkable, and surpasses the pure diet intervention;Unifies the diet intervention in the acupuncture intervention's foundation,the effect is better.The acupuncture union diet intervention may repair the blood vessel bast which suffers injury to reverse as a result of the insulin resistance,its mechanism possible with its adjustment unbalanced in vivo oxidation -oxidation resistance system,improvement blood rheology,reduces ET which,TXB2 the model elevates,elevates NO,6-Keto-PGF1αto concern.â‘¢Existence question:This model big mouse aorta bast has not seen the accurate artery gruel type mottling formation,aorta VEGF expresses in each group has not had the remarkable difference,possible with to make the mold time to concern.Next step research direction:How continues to consummate to appraise the IR model the blood vessel bast appraisal function target,from genomics,the protein group study or a higher stratification plane further thoroughly discusses the IR related complication the pathogenesis,the discussion acupuncture and other methods prevents and controls IR and the related complication function mechanism, and further optimizes the therapeutic schedule.