Effect Of KaiweiJinshitang Ultramicro-drug Piece On Gastrointestinal Hormone Of Spleen Deficiency

PartⅠClinic research on Spleen deficiency treated by Kaiwei Jinshitang ultramicro-drug pieceObjective:To evaluate the clinical effect of Kaiwei Jinshitang(KWJST) ultramicro-drug piece(UMDP) on Spleen deficiency,and the gastrointestinal hormone changes.Method:90 patients with Spleen deficiency were divided randomly into three groups,treated with one dose and half dose of KWJST UMDP and Jianwei Xiaoshi Tablet(JWXST) respectively,two weeks for one period of treatment,the serum gastrin(GAS),somatostatin(SST) and motilin(MTL) were selected as evaluating items.Results:1.The total effect rate of one dose and half dose of KWJST UMDP were 86.67%and 83.33%respectively and the clinic efficacy were equal.The effective rate of KWJST UMDP was higher than that of JWXST(P＜0.05).2.One dose and half dose of KWJST UMDP and JWXST could raise serum GAS and MTL,while reduce SST,and there were no significant difference among them.Conclusion:KWJST UMDP could improve the symptom of Spleen deficiency, the half dose group equals to one dose group.KWJS UMDP could prompt GAS and MTL,as well inhibit SST secretion.PartⅡThe effect of KWJST UMDP on rats' gastric juice secretionObjective:To study the effect of KWJST UMDP on the gastric juice secretion in normal rats and Spleen deficiency rats induced by reserpine.Method:In order to find out the best dose,random comparing design applied, and JWXST selected as positive contrast drug,one dose,half dose and one fourth dose of KWJST UMDP,and traditional decoction of KWJST respectively were used to observe the effect of the above materials on the normal SD rats' gastric juice secretion.And the effect of KWJST UMDP on the gastric juice secretion in Spleen deficiency rats induced by reserpine was also evaluated.Results:1.Comparing to normal group,JWXST and traditional decoction and different doses UMDP of KWJST could increase rats gastric juice secretion, decreasing gastric juice PH value,increasing free acid and total acidity and output of total acid,as well pepsin activity.As for the increasing amplitude,half dose of KWJST UMDP equaled to one dose,and was higher than one fourth dose(P＜0.05). While there were no significant difference among traditional decoction and half dose of KWJST UMDP and JWXST.2.Comparing to Spleen deficiency group, the gastric juice,free acid,total acidity and output of acid were lower in JWXST, traditional decoction and half dose of KWJST,as well as pepsin activity,while the higher PH value in later three groups.And for the above items,there were no significant difference among the later three groups.Conclusion:KWJST UMDP has double effects on rats' gastric juice secretion.1. It could promote normal rats' gastric juice and acid secretion,and increase pepsin activity.With the higher dose,the higher effects appear,and half of dose has best effects.2.It could also inhibit gastric juice and acid secretion and pepsin activity in Spleen deficiency rats induced by reserpine.PartⅢThe effect of KWJST UMDP on Spleen deficiency rats' GAS,SST and MTL content in tissue and serum respectivelyObjective:To explore the effect of KWJST UMDP on GAS,SST and MTL content in tissue and serum of normal rats and Spleen deficiency rats induced by reserpine.Method:60 SD rats were randomly divided into normal contrast group,normal group with KWJST UMDP,Spleen deficiency group,Spleen deficiency treated with KWJST UMDP,traditional decoction group,JWXST group.Spleen deficiency group were induced by reserpine,and normal contrast group with normal saline.Then normal saline,half dose of KWJST UMDP and JWXST intragastric administration for two weeks,the tissue and serum GAS,SST and MTL were assayed respectively by radioimmunoassay(RIA).Results:1.The tissue and serum GAS and MTL in the normal group with KWJST UMDP were higher than those in normal contrast group significantly (P＜0.01),while the SST was lower in the normal group with KWJST UMDP(P＜0.05).2.Both of the tissue and serum GAS in Spleen deficiency treated with KWJST UMDP,traditional decoction of KWJST and JWXST,were lower than those in Spleen deficiency group,while SST and MTL were higher than those in Spleen deficiency group.3.Among the groups,the tissue and serum GAS were lower in Spleen deficiency treated with KWJST UMDP than those in traditional decoction of KWJST and JWXST,while the SST and MTL were higher.Conclusion:KWJST UMDP has double regulating function for rats' gastrointestinal hormone.1 It could stimulate normal rats with more GAS and MTL secretion,inhibiting SST secretion.2 It could raise the tissue and serum SST and MTL and reduce GAS level in the Spleen deficiency rats induced by reserpine. It shows that KWJST UMDP could regulate the inordinate gastrointestinal hormone for the normal level.PartⅣThe regulation of KWJST UMDP on normal rats and Spleen deficiency rats' GAS mRNA and SSTR mRNA expression in sinuses ventriculi mucosaObjective:To observe the effect of KWJST UMDP on normal and Spleen deficiency rats' GAS and SSTR mRNA expression in sinuses ventriculi mucosa, and explore the molecular mechanism of regulating gastrointestinal hormone.Method:RT-PCR was used for GAS mRNA and SSTR mRNA expression in rats' sinuses ventriculi mucosa.Results:1.KWJST UMDP could up-regulate GAS mRNA expression in sinuses ventriculi mucosa of the normal rats,while weakening its expression in the Spleen deficiency rats.2.KWJST UMDP could weaken SSTR mRNA expression in sinuses ventriculi mucosa of the normal rats,while up-regulating its expression in the Spleen deficiency ratsConclusion:KWJST UMDP could regulate gastrointestinal hormone by way of up-regulating GAS mRNA and down-regulating SSTR mRNA expression in normal rat,up-regulating SSTR mRNA and down-regulating SSTR mRNA expression in Spleen deficiency rat.And it is the base for regulating gastric juice and acid secretion,which is the molecular mechanism of KWJST UMDP treating Spleen deficiency in a way.