| 1.According the systematic sorting of the literature and magazine to explore the regulation of syndrome differ classification that each docter cures incomplete paraplegia using acupuncture。(1) The regulation of syndrome differ classification in Traditional Chinese Medicine:Consulting 62 literature of Chinese medicine,western medicine,combine traditional Chinese and western medicine in 2000-2005 years,the dilectical classification in incomplete paraplegia can be divided into syndrome of Qi - stagnated and blood - stasis,syndrome of deficiency of spleen- yang and kindey- yang,syndrome of deficiency of liver- yin and kindey- yin.(2) The regulation of clinic medicine usage:Statisticsed a total amount,the frequency of each kind of medicine used,the number combined and the medicine used in the square total amount,the medicine categorized,the usage frequency of each medicine,the main method of treatment with the medicine and the usage of all prescriptions.Results:Use 55 prescriptions,the tallest usage frequency is a kind of prescription that can promote blood circulation and remove blood stasis in these prescriptions。Second is the kind of prescription that can increase blood。This can be explained that it is the most important that using prescription that can promote blood and increase the blood in the treatment of incomplete paraplegia。In the 55 prescriptions,the theory cure namely particularly the disease is 18,37 prescriptions are the ones that based on differentiation of symptoms and signs。The function of all the two kinds are mainly promoting blood circulation and removing blood stasis.The total number of usage frequency more than 10 times is about 25,in which the tallest usage frequency includes danggui,niuxi,shudi, tubiecong,lujiaojiao。Thus,it is the most that the usage of those prescriptions that can promote blood and increase blood in the treatment of incomplete paraplegia."promoting blood and increasing blood, calefaction positive gas(Du meridian) and stimulate paralysis" is the key method in the treatment of incomplete paraplegia。(3) The regulation of syndrome differentiation of acupuncture:we referred to 58 dissertations,summarizing six laws about how to select aperture:â‘ to take Du meridian at first when treating the impotence syndromes;â‘¡to take Huatuo Jiaji acupoint as supplement;â‘¢to take Wuzhang acupoint and Ge acupoint as adjuvant;â‘£the meridian of urinary bladder and gallbladder can also be used as assistance;⑤to take Yangming meridian when treating the impotence syndrome;â‘¥to take Jin acupoint as usual when treating the impotence syndrome.The summarization of 13 laws about how to use the method of acupuncture and moxibustion:â‘ the method of pricking Du meridian;â‘¡to choose acupoints by matrix form;â‘¢the method of stimulating nerve stem;â‘£the method of pricking Huatuo Jiaji acupoint;⑤to use ten-joint electrical needles;â‘¥the method of awaking brain and opening aperture for acupuncture;⑦therapy of needles-in-line;⑧stimulating the nine-needle acupoint of certain part§ion;⑨the method of needle-movement;â‘©the method of long needle penetrating the acupoint;(11)to take seven-star needle for pricking;(12) moxibustion;(13) the method of acupoint-injection.2.The clinic study of "liver and kidney-yin-weak "syndrome of incomplete paraplegia with acupuncture.The standardization clinic study was explored on the regulation of syndrome differ classification incomplete paraplegia with curative effect observing of many hospitals.Collectivity curative effect and condition of urinate function improving were observed with acupuncture on Du meridian and nerve trunk,acupuncture on Du meridian,stimulating on nerve trunk in the frist hospital of Hunan college of tradition Chinese medicine,The hospital of hunan tradition Chinese medicine academy, The fourth hospital of Changsha.The content of neuropeptides(B-EP,DYNA,AVP) in blood and cerebrospinal fluid before and after therapy,to explore mechanism of acupuncture on paraplegia.Result:through the observation of 178 patients,the result is all the three therapies can treat traumatic paraplegia,but the therapy of Du meridian and nerve trunk is better than others,which can get to 70%of recovery rate on incomplete paraplegia and 100%of removing rate of catheter.It can significantly shorten period of treatment,improve curative effects,decrease cost,so it is an easy,safe,effective and recommendable therapy of acupuncture.The content of neuropeptides changed distinctly before and after therapy.The effect of acupuncture is related to decreasing the content of DYNA,B-EP,AVP.3.The experiment study on the regulation of syndrome differ classification incomplete paraplegia of rabbit with acupuncture.Three syndrome phase of incomplete paraplegia and the essence of effect of acupuncture were explored in the animal experiment study.The animal experiment adopts the pressing model of rabbit,adopts photoelectrical scope to observe spine and positive expression of neuropeptides,NGF and VEGF.Result:The status that Spinal cord was scathed to bleeding and dropsy was the best severity were same as that the pinnacle status of positive signal number of VEGF within syndrome of Qi - stagnated and blood- stasis afer the spinal trauma in 6 hours.Taking acupuncture one time after the spinal trauma can maintein the positive signal number of VEGF,reduce the dropsy,bleeding and effusion,maintein the normal density nucleus,maintain the functional area and number of mitochondria, can promoe energy metabolismof mitochondria and neuron recovery. Acupuncture can promote the expression of BFGF to regenerate the traumatic neuron,can clean the inflammatory cells and inhibit the release of NGF(the increase of neurotrophins is important to defence and repair of impaired neuron).Morever,acupuncture can adjust the level of some neutopeptides,can carry them into neuron to participate in the repair of tranmatic spinal cord:adjust the content of AVP,B-EP,accelerate the degeneration and diffusion DYNA and the combination with receptor so as to alleviate the secondary impair of spinal cord.
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