Study Of Mechanism Of Yizhi Wuhai Capsule On Vascular Dementia

Objective:To investigate the Yizhiwuhai capsules treat animal model of vascular dementia study learning and memory in the hippocampus of the intervention and of Ach, AchE, Glu, Gly, GABA, Asp impact; of monoamine neurotransmitters in the cerebral cortex 5-HT, DA, NE impact; of related vasoactive substances ET, CGRP, TXB2,6-Keto-PGF1αimpact; on Hemorheology; on the effects of ChE in blood, and then to explore the Yizhiwuhai capsules in treatment of vascular dementia of the mechanism of action.Methods:The establishment of an improved bilateral ligation of carotid artery (CCA) of the result of ischemia-reperfusion rat model of vascular dementia, Mailuoning stasis combined ischemia-reperfusion rat model of vascular dementia, senile rat model of essence and memory deficiency mouse model of access barriers, obstacles to the consolidation of memory in rat model, loss of memory in mice model of reproduction, with Morris water maze method, platform method, to avoid detection dark spatial learning and memory behavior of the indicators; measured by capillary electrophoresis in the hippocampus Glu, Gly, GABA, Asp content, ELISA method in the Ach content of the hippocampus, the hippocampus in the Determination of the content of AchE, fluorescence spectrophotometric determination of the cerebral cortex 5-HT, DA, NE content. Blood measured by radioimmunoassay ET, CGRP, TXB2,6-Keto-PGF1αcontent. Kidney hydrocortisone injection to establish a mouse model to determine their swimming time, organ index. Hydergine used for positive control drug.SPSS11.5 conducted using one-way ANOVA statistical analysis of experimental results.Results:The model and the control group, Yizhiwuhai capsule high dose of acetylcholine in hippocampus were significantly increased compared with the model group significantly different (p<0.05); middle-and high-dose group of vascular ischemia-reperfusion dementia rat model of the role of NE significantly increased (P <0.05); the treatment group increased CGRP,6-Keto PGFla significant role (P< 0.05); middle-and high-dose group TXB2 significantly lower (P<0.05); However, lowering the effect of ET was not significant (P>0.05); of drug-treated to reduce whole blood low-shear reduced viscosity, a relatively low shear whole blood viscosity (P<0.05), plasma viscosity (P<0.05); Reduce hippocampal Glu, Asp content increased Gly mass (P<0.05), of drug-treated to extend the swimming time in mice Kidney; study of the treatment group behavior, such as detection of the escape latency differences were significant (P<0.05).Conclusions:The Yizhiwuhai capsule with Bushen Yizhi, Tongmai active through the kidney essence, Qualcomm stasis, and phlegm, pass the meridian, Shu Qi stagnation, so that a premium essence, brains were raised, was of blood, turbidity can be eliminated, too Xuanfu open, pass a context, a resumption of intelligence. Animal models can improve.spatial learning and memory functions, improve the animal model of intelligence, which may be as follows:increasing seahorse Ach content and the cerebral cortex NE content, simultaneously, may reduce in the blood the ChE content, the maintenance seahorse neuron information transmission and the choline can the nerve function integrity, reduces animal model seahorse Glu, the Asp content, enhances Gly the content, reduces the amino acid toxicity to seahorse's damage, thus maintenance nervous tissue function integrity.Adjustment blood vessel active material CGRP and ET, TXA2 and the PGI2 content, namely elevates CGRP, the PGI2 content, reduces ET, the TXA2 content; The expansion blood vessel, the improvement blood circulation, guaranteed the brain organizes because the blood supply, reduces the brain organization to lack the damage which the anoxemia creates; Enhances the blood the rheological property, thus improves the blood which the brain organizes for with the oxygen to supply.