| The abnormal change of monoamine neurotransmitters is an essential factor in the process of acute cerebral ischemia and reperfusion. The monoamine neurotransmitters promote calcium overload, contracting vessels, releasing of free radicle and excitatory amino acid. These factors initiate series of pathological effects, which lead to neuronal apoptosis and necrosis.This study observes the changes of momoamine transmitter and morphology after cerebral ischemia-reperfusion in rats dynamically. Using the clinical effective drug-Xinnaoshutong capsule to treat cerebral ischemia-reperfusion injury, to observe changes of monoamine transmitter. Discuss the possible protective mechanism of Xinnaoshutong capsule on cerebral ischemia- reperfusion injury in rats.AIM : To observe the dynamic changes of monoamine neurotransmitters and morphology in cerebral cortex at different times of reperfusion after 1.5 hours of ischemia in rats. To discuss the curve of the monoamine neurotransmitters concentration in acute cerebral ischemia-reperfusion. To study protective effects and mechanisms of activate blood, resolve stasis and free the collateral vessels medicine—Xinnaoshutong capsule on cerebral ischemia and reperfusion injury in rats.Methods:1 . Establishment a high performance liquid chromatography(HPLC) method of monoamine neurotransmitter. Animal model of focal cerebral ischemia-reperfusion is the thread occlusion technique induced middle cerebral artery occlusion in rats. Rats were divided into eight groups randomly: sham group, and 45min, 1h, 3h, 6h, 12h, 24h, 72h of reperfusion respectively after 1.5h of ischemia by transient occlusion of middle cerebral artery. Determinate concentrations of the monoamine neurotransmitters norepinephrine epinephrine dopamine, serotonin and dopamine metabolite 4-hydroxy-3-methoxy-phenylacetic acid simultaneously in cerebral cortex of different intervals of cerebral reperfusion after 1.5h focal cerebral ischemia. Observing morphological changes of cerebral cortex by HE staining.2.Animal model of focal cerebral ischemia and reperfusion is the thread occlusion technique induced middle cerebral artery occlusion in rats. Rats were randomly divided into three groups: sham group, 24h of reperfusion after 1.5h of transient cerebral ischemia model group, Xinnaoshutong capsule treatment group. Observing morphological changes of cerebral cortex by HE staining and concentrations of the monoamine neurotransmitters by HPLC.Results:1.The established HPLC method of monoamine neurotransmitter is simultaneous and rapid determination. This method has a high degree of precision and recovery rate.2.The trend of change of morphology is gradual aggravation by the extension of reperfusion intervals. The pathological injury has reduced at reperfusion 72h.3.Different degrees of monoamine neurotransmitters disturbance were observed at different times of reperfusion after 1.5h of ischemia. The content of E increased at first and then decreased back to sham level,which peaked at 6h of reperfusion;the volume of DA has an increased tendency,which peaked at 24h of reperfusion;the content of HVA and 5-HT increased which peaked at 45min,6h of reperfusion respectively;the level of DA/HVA dropped significantly.4.Xinnaoshutong capsule has the following effects on reperfusion after cerebral ischemia: reducing pathological injury and content of E, DA and 5-HT, increasing level of DA/HVA, and an decreased tendency of HVA .Conclusion:Focal cerebral ischemia and reperfusion induces different degrees of brain pathological injury, and in the dynamic process monoamine neurotransmitters are disturbed. Xinnaoshutong capsule could mitigate the change of pathological injury, and the possible protective mechanism of which is to improve disturbed neurotransmitters...
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