| As an ultrasensitive analytical technique, surface-enhanced Raman scattering (SERS) has been attracted increasing attention in pharmaceutical molecules science. SERS-based methods have salient advantages of high sensitivity, photostability and spectral multiplexing over conventional methods, based on which we successfully developed two SERS-based detection methods for pharmaceutical molecule-Human Serum Albumin (HSA) complex. The major contributions of this work are as follows:(1) Solid-enhanced substrate fabricating and HSA-mediated orientation of protoberberine alkaloidsWe developed a new simple method by depositing silver colloid on modified glass, which is an excellent SERS-active substrate for protoberberine-HSA complex. The proposed method is facile and sensitive, and the most important thing is after recognition, we can determine different pharmaceutical molecules by SERS, which is accessible for identifying the orientation of pharmaceuticals.(2) The orientation of flavonoids influenced by HSA in aqueous solutionsFluorescence and ultraviolet-visible spectra are basic and efficient methods to identify the recognition between flavonoids and HSA, while the information about the flavonoid's structure is limited. The SERS spectra are obtained in silver colloid in physiological conditions, then the various orientation based on different structure will be embodied.(3) Based on physiological SERS substrate to detect the flavonol-HSA complexThe flavonols are quite different from flavonoids not only in physical and chemical properties, but also in physiological activities. The silver nanoparticles are used as the SERS substrate in pH 7.4 aqueous solutions, and then vibrational information of flavonols will be probably displayed in their SERS spectra with remarkable enhanced reproducibility. When in the flavonol-HSA complex, the SERS intensity of different vibrations will stand for the changing orientation of flavonols.
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