The Association Between Chronic Stress And Blood Pressure, Blood Glucose, Blood Lipids, Cortisol, Epinephrine And Norepinephrine In Serum, 5-HTTLPR Polymorphism

Objective:1. To assess the effect of the chronic stress on blood pressure, blood glucose and blood lipids.2. To explore the relationship between the chronic stress and cortisol, epinephrine and norepinephrine in serum.3. To investigate the association between the chronic stress and the polymorphism of 5-hydroxytryptamine transporter-linked promoter region.Methods:We used cross-sectional study with the 847 soldiers stationed in the gobi. Trait Coping Style Questionnaire, Perceived Social Support Scale and Symptom Checklist-90 were adopted to evaluate the stress state of the participants. Systolic pressure, diastolic pressure, fasting plasma glucose, oral glucose tolerance test 2h plasma glucose and the concentrations of TC, TG, LDL-C, HDL-C in serum were measured. The concentrations of cortisol, epinephrine and norepinephrine in serum were also measured. The genotypes of the polymorphism in 5-HTTLPR were determined by using polymerase chain reaction and agarose gel electrophoresis. The data were statistically treated.Results:1. The scores of SCL-90 of the participants were significantly higher than the norms of SCL-90 of Chinese (P<0.01). NC was correlated with FPG, OGTT2hPG, TC, lgTG and LDL-C (P<0.05), PC was correlated with FPG (P<0.05), and SCL-90 was correlated with SBP, FPG, OGTT2hPG, TC, lgTG and LDL-C (P<0.05). Multiple stepwise regression analysis showed that NC was an influencing factor on TC and LDL-C (b= 0.013,0.008), that PC was an influencing factor on FPG (b=-0.006), and that SCL-90 was an influencing factor on SBP, OGTT2hPG, TC, lgTG and LDL-C (b=0.014,0.002, 0.002,0.001,0.001).2. As to COR, E and NE, there was no significant difference between the participants and the normal people. SCL-90 was correlated with COR, E and NE (P< 0.05), and NC was correlated with COR and E (P<0.05). Multiple stepwise regression confirmed that SCL-90 and NC was influencing factors on E (b=0.054,0.385).3. The allele of 5-HTTLPR had strong genetic relationship with the scores of NC and SCL-90 (X2=21.232,30.359, P<0.05). There were significant differences in NC and SCL-90 between the two groups (P<0.05), the stress state in the participants with S/S genotype was statistically higher than that in those with S/L or L/L genotype.Conclusions:1. The stress state in the participants is significantly higher than that in normal people. The chronic stress may be a risk factor of hypertension, hyperglycemia and hyperlipemia.2. The chronic stress may be related to the increase of E in serum.3. The allele S is more sensitive to stress than the alleve L, and the individuals with S/S genotype may suffer more intense stress reaction than those with S/L or L/L genotype.