Correlation Studies Based On Different Sources Of The Skullcap Material Base And Its Efficacy School

Clarify the material basis of Traditional Chinese Medicines Chinese medicine has always been a hot issue researchers. On the one hand, the material basis of efficacy of Chinese medicine to promote the modernization of Chinese medicine is one of key problems. On the other hand, to find a single ingredient formulation guidelines and the overall effectiveness of various research methods and direct the core of traditional Chinese medicine theory is associated. Therefore, the appropriate use of modern scientific theories and technological results, consistent with characteristics of Chinese medicine theory of the feasibility study on their own ideas and methods, to clarify specific efficacy of Chinese medicine and its corresponding material basis for the acting mechanism of urgent problems.Scutellaria baicalensis for the Labiatae plant dry root of Scutellaria baicalensis Georg, only contained in the "Shen Nong's Herbal Classic", a heat drying dampness, purging fire detoxification, bleeding the effect of tocolysis. The skullcap ingredients, has been extracted to flavonoid-based, more than 60 chemicals, including baicalin, baicalein, baicalin, wogonin, baicalin new factors, are also based on baicalin content higher being "Pharmacopoeia" as the detection of baicalin quality standards.The main components from Scutellaria pharmacological research results, the pharmacological effects of the chemical composition of some of the same, but their effect is not completely overlap, there are complementary aspects. Different sources of content of main component baicalin significantly different, as the genuine medicine is widely recognized in Chengde, Hebei baicalin, baicalin content in comparison with other producing less, but their antipyretic, anti-inflammatory efficacy is so better than other parts of skullcap. Therefore, only baicalin content to evaluate the skullcap of quality might be limited, baicalin content level could not be fully representative of baicalin effect, skullcap, in addition to baicalin things might otherwise play a key role in the active ingredients, to skullcap reasonable proportion of the major active components as the embodiment of the quality of baicalin efficacy studies may reveal the role of the material basis of its efficacy, the efficacy of Chinese herbal medicines for other studies for referenceThe topic of different sources of baicalin chemical composition, extraction, determination and the active ingredients of pharmacological aspects to be investigated.Objective:To clarify the characteristics of baicalin quality, efficacy of material basis for quality assessment and clinical baicalin and production of drugs provide the scientific basis for quality and artificial cultivation of Scutellaria theoretical basis for product quality control.Methods:In this study, under the guidance of TCM theory to Chinese pharmacology as the main research methods, combined with Chinese chemical and chemical composition analysis for the heat dampness skullcap, Xiehuo two detoxification effect, to carry out heat drying as well as anti-inflammatory, anti-virus, fever and other pharmacodynamic test, chemical analysis by traditional Chinese medicine, Fingerprint, database integration and information processing methods based on different sources of material and the efficacy of Scutellaria Correlation.Results:1. Antiinflammatory pharmacodynamic test:the extracts from different areas all of the mice ear swelling compared with the model group were significantly different (p <0.05), in Hebei, Shanxi alcohol alcohol group and the difference between the model group is particularly significant (P<0.01), between the two groups was no significant difference (p> 0.05); different origin groups the extracts inhibit the rat paw edema different point in time, the overall efficacy of 22 compared with the model group were significantly different (p<0.05), Hebei ethanol extract compared with the model group is particularly significant difference (P<0.01).TDA Scutellaria Hebei ethanol group and the mice ear swelling compared with the model group were significant differences(P<0.01), ratio of group and alcohol group Hebei no significant difference (p> 0.05); TDA Group skullcap and Hebei extract group rat paw swelling compared with the model group were significantly different overall (p<0.05), Hebei alcohol extraction rate of rat paw edema model is particularly significant difference between groups (p<0.01), ratio of group and Hebei alcohol group had significant differences (p<0.05).Different years the extracts of mice ear swelling rate and the model group,22 were significantly different (p<0.05), each rat paw edema rate and the model group, overall there was a significant difference (p<0.05) have to biennial Scutellaria group and model group difference was most significant (P<0.01).Different areas in the same area cultivated Scutellaria all of the mice ear swelling model group 22 were significantly different (p<0.05), in Hebei Province, biennial controls were the most significant (P<0.001); the group extract inhibits rat paw edema different point in time, the overall efficacy compared with model group,22 significant differences (p<0.05).2. Antipyretic pharmacodynamic test:the extracts of different age groups antipyretic effect compared with model group,22 were significantly different in general (p<0.05), biennial general Scutellaria rats, body temperature changes and year group, three significant difference between groups (P<0.5), year group, three rats, body temperature change was not significant difference (P> 0.05).Cultivation of different origin in the same area of each group of Scutellaria antipyretic effect compared with the model group 22 overall significant difference (p<0.05), with biennial Hebei Province, the most significant difference between groups (p<0.01).3. Antiviral-effect test:the extracts of different age groups compared with the model group mortality was significant difference (P<0.01), the extract was no significant difference between the 22 (P> 0.05). Lung index, the Hebei Chengde skullcap biennial and annual no difference (P> 0.05), and three students were significantly different (P <0.05), annual and three-year-no difference (P> 0.05).Different areas in the same area cultivated Scutellaria the mortality compared with model group, in addition to Dalian three groups significantly different (P<0.05), the other groups compared with model group there was a significant difference (P <0.01), the extraction matter between two groups showed no differences (P> 0.05). Lung index in two groups of Hebei, Dalian three years compared with model group, there were significant differences (P<0.05), the other extracts compared with model group, there was a significant difference (P<0.01), the extract difference between groups was not significant (P> 0.5).4. Clearing heat and dampness pharmacodynamic test:fed with E. coli, all the rats body temperature, stool frequency increased, there was pulpy form, some rats appeared swollen anal congestion. All of the rats curled lying lazy move, towering hair apparent, especially on dark coat without Ze, compared with normal weight much slower growth trend. Body temperature of rats in all treatment groups declined faster than the control group, decreased stool frequency, rectal swelling less. Medication to 4 days, the model control group eating capacity and the normal control group, there was a significant difference (P<0.01), while the treatment group differences in dietary content did not change (P> 0.05). SOD activity, the extracts from different sources SOD activity of 22 group showed no significant difference (p> 0.05). MDA levels, the extracts from different sources MDA level of 22 groups showed no significant difference (p> 0.05).5. Anti-inflammatory efficacy of the active ingredient composition and effect relationship between the spectrum of research:Hebei ethanol extract of different retention time stability of chemicals in the 1.8%-2.98%, and less than 3%, good reproducibility. Grey relational analysis by ethanol extract of Scutellaria Hebei active ingredient, ear swelling of mice associated with the material greater than 0.74 in order to retain the material time was 12.0min> retention time 70.1min material> retention time 58.5min material>baicalin>wogonin.The role of anti-rat paw swelling, the correlation is greater than 0.74 in order of the material retention time 70.1min material> Wogonin> retention time 72.9min material> retention time 58.5min material> retention time was 43.8min material> baicalin> retention time 8.5min material> retention time 39.9min material> baicalin.Conclusion:1 Different origin skullcap extract on rats have a certain heat treatment can improve damp the operation of the spleen and stomach function in rats, rats reduced body temperature, improve immune function in rats, but rather the origin efficacy.2 Different origin skullcap extract on xylene-induced mouse ear swelling model, dry yeast induced rat paw swelling models have better anti-inflammatory effect, Hebei alcohol extract the best effect of inhibition of acute inflammation.3 Grey relational analysis can be a more accurate assessment of fingerprints and the correlation between efficacy, spectrum efficiency for the establishment of Chinese Studies, with research methods, has some practical value.4 Ratio of the active ingredient of Scutellaria matter (baicalin:wogonin:baicalin= 0.68:0.47:11.9) on xylene-induced ear swelling and inflammation in a better inhibition effect with the alcohol extract of Scutellaria Hebei rather, in the represent a significant anti-inflammatory efficacy of Scutellaria material basis; ratio of material (wogonin: baicalin:baicalein= 0.47:11.9:3.8) on yeast-induced paw edema also has some inhibitory effects, but the detection Hebei efficacy at various time points were lower than the ethanol extract of Scutellaria, ratio of components may be outside than other active ingredient is still inhibited rat paw edema and swelling of the important material basis for efficacy.5 Years of the extracts of different yeast-induced fever on the model showed a better antipyretic effect, antipyretic effect, but to play point in each group are different; on xylene-induced mouse ear swelling model, dry yeast-induced Foot swelling models have better anti-inflammatory effects, the experiments were the best effect of Hebei biennial skullcap; on mice infected with influenza virus FM1 has better protective effect of the death, can reduce the lung index.6 Introduction to Dalian from different areas of two years, three years skullcap extract on the dry yeast-induced fever model at different time points show some antipyretic. On mice infected with influenza virus FM1 has better protective effect of the death, can reduce the lung index. On xylene-induced mouse ear swelling model, dry yeast induced rat paw swelling model showed a certain inhibition of inflammation, but dry yeast-induced paw edema inhibition, the efficacy of testing anti-inflammatory drugs different point in time.7 Different years, different areas and ex situ cultivation of Scutellaria pharmacodynamic comparison of horizontal and vertical, Hebei Cheng de biennial skullcap good antipyretic, anti-virus, anti-inflammatory effects, in order to confirm the genuineness of Scutellaria and cultivated to provide a theoretical support.8 Different pharmacological effects of Scutellaria corresponding material basis for efficacy of different, the subject of research ideas to a certain extent, quality standards for follow-up study of Scutellaria provides the reference and research approaches.