Shenqifuzheng Injection Solution The Molecular Mechanism Of The Synergistic Effect Of Chemotherapy

1 ObjectiveTo explore effects of ShenQiFuZheng injection on gene expression profile of liver metastasis cancer in mice suffering chemotherapy.2 Methods2.1 Constructing mouse model of liver metastasis cancerSelecting BALB/c mice, establish the experimental models by cutting spleens after injecting C26 mouse colon cancer cells in them.2.2 Group and Administration20 Mouse models of liver metastasis cancer were set up and then randomly divided into two groups:5-Fu group, Shenqi Fuzheng Injection+5-Fu group.2.3 ObservationMice were weighted every day after being inoculated tumor cells. Observe the activity, appetite and the development of tumors. Tumor-bearing mouse were sacrificed at the thirteenth day. Tumor tissues were weighed and reserved with liquid nitrogen, then prepare the nucleic acid, detect the gene chips and analyse the genes with bioinformatics.3 Results3.1 All mouse models were set up sucessfully;3.2 Gene expressionShenQiFuZheng injection could up-regulate 13 genes and down-regulate 282 genes.4 ConclusionsShenQiFuZheng Injection can affect Several signal pathways in the tumor, including oxidative phosphorylation, purine metabolism, pyrimidine metabolism, VEGF signaling pathway, adherens junction, focal adhesion.