The Theoretical And Experimental Study On The Bushen Jiedu Tongluo Act Improving Chronic Renal Failure Rat

Objective Probing the basic pathogenesis of chronic renal failure, its therapeutic methods and the composition of herbs under the direction of traditional chinese medicine theory combined with collateral disease theory, then through animal experiments to study the mechanism of the Bushen Jiedu Tongluo act, so as to effort warranty of the experiment for clinical application.The text was divided into three parts:theory probing, experiment al study and conclusion.Theory probing 1The basic pathogenesis of chronic renal failure is asthenia of kidney, venation obstruction and toxic factors accumulating in tissue. (1)The primary aspect of chronic renal failure is asthenia of kidney: kidney storing essence, being the organs of the yin and yang and trials of life.We call it"a priori of the present". Kidney deficiency and it vulnerable to the attack of internal-external influences, So evil can infiltrate by taking advantage of the other side's unpreparedness, hurt Yin and accumulate poison for a long time which could obstruct kidney network, it affect various physiological function and structure of the kidney which lead to the incidence of chronic renal failure. (2) Venation obstruction and toxic factors accumulating in tissue are the pathological basis of chronic renal failure:Kidney is a priori of the present. On the one hand, insufficiency of kidney-qi cause the function of producing microscopic materials reduced, the kidney-essence and blood decreased, as that the kidney network dystrophy cause the function of communicating exterior and interior, infiltrating blood and fluids, and excreting body wastes reduced which lead kidney network malnutrition and collateral disease generate. On the other hand, it cause the power of blood circulation down which lead turbid phlegm, blood stasis and pathogenic toxin obstruct the kidney network. While the renal collaterals is the key and the hub of qi and blood for renal nutrition.If renal collaterals is clear, qi and blood is sufficient, then the kidney function was to support the healthy and vigorous; if renal collaterals is not clear, qi and blood is deficiency, kidney failure is the failure to support. (3) The two which is asthenia of kidney and which is venation obstruction and toxic factors accumulating in tissue interact, reinforce each other:chronic renal failure disease process is various reasons causes kidney damaged, so we lost two division, San Jiao damaged, the function of separating the clear from turbid decreased, damp turbidity and uremia can not be eliminated from the body while accumulate in the body which lead to venation obstruction. In turn, toxic factors accumulating in tissue injury righteousness, block air machine, increase the network weakness, so on and so on, adding condition.2. The main treatment of chronic renal failure is nourishing the kidney as well as removing toxic materials and dredging collaterals according to the pathogenesis:TCM establish treatment principles according to etiology, pathogenesis and pathological changes. The basic pathogenesis of chronic renal failure is asthenia of kidney, venation obstruction and toxic factors accumulating in tissue, so the main treatment of chronic renal failure is nourishing the kidney as well as removing toxic materials and dredging collaterals. Relapse of chronic renal failure cause that its treatment focuses on both the root and branch aspects, supporting healthy qi and removing pathogenic factors. So as that we can tonify deficiency, activate the blood, dissolve phlegm and detoxication.3 Prescription basis and recipe analysis:Prescription of Bushen Jiedu Tongluo act representative for the Bushen Jiedu Tongluo recipe which consisting of:astragalus 30g, epimedium 20g, fructus corni 15g, salvia miltiorrhiza 30g, earthworm 15g, honeysuckle 15g, rhubarb 6g. Meanwhile, astragalus and epimedium is monarch drug for reinforcing kidney and qi. Fructus corni, salvia miltiorrhiza, earthworm and honeysuckle is ministerial drug for reinforcing kidney and qi, removing blood stasis, expelling miasma, freeing channel. Rhubarb is assistant and guide for clearing heat and expelling miasma, promoting blood circulation by removing blood stasis. All side effects for tonifying qi of the kidney, clearing away heat and toxic material, activating blood and dredging collaterals.Experimental study Methods:The chronic renal failure rat models were caused by adenine according to literature method. The SPF-class male Wistar rats were randomly divided into two groups:normal control group and model group. After the success of modeling, the rats in model group were randomly divided into three groups:model control group,Niaoduqing group and Bushen Jiedu Tongluo group. After administration respectively,we observed BUN,Scr,transforming growth factorβ1,collagen typeⅠ,bone morphogenetic protein 7,TGFβ1mRNA,col-ImRNA and BMP-7mRNA. Results:1BUN and Scr:The points of BUN,Scr in the model group and treatment groups was higher significantly compared with the normal group(P<0.01).The points of BUN,Scr in the Niaoduqing group and Bushen Jiedu Tongluo group was decreased compared with model control group(P<0.01). The points of BUN in the Bushen Jiedu Tongluo group was decreased compared with Niaoduqing group (P<0.05), while the points of Scr between them had no significant difference(P>0.05).2Col-I protein: The expression of col-I in the model group, Niaoduqing group and Bushen Jiedu Tongluo group was increased compared with the normal group(P<0.01). The expression of col-I in the Niaoduqing group and Bushen Jiedu Tongluo group was decreased compared with the Model group (P<0.01). Bushen Jiedu Tongluo group and Niaoduqing group had significant difference (P<0.01, P<0.05).3 TGF-β1 protein:The expression of TGF-β1 in the Model group, Niaoduqing group and Bushen Jiedu Tongluo group was increased compared with the normal group(P<0.01). The expression of TGF-β1 in the Niaoduqing group and Bushen Jiedu Tongluo group was decreased compared with the Model group (P<0.01, P<0.05). Bushen Jiedu Tongluo group and Niaoduqing group had significant difference (P<0.01, P<0.05).4 BMP-7 protein:The expression of BMP-7 in the Model group, Niaoduqing group and Bushen Jiedu Tongluo group was decreased compared with the normal group(P<0.01). The expression of BMP-7 in the Niaoduqing group and Bushen Jiedu Tongluo group was increased compared with the Model group (P<0.01). Bushen Jiedu Tongluo group and Niaoduqing group had significant difference (P<0.01, P<0.05).5Col-ImRNA:The expression of Col-ImRNA in the Model group, Niaoduqing group and Bushen Jiedu Tongluo group was increased compared with the normal group(P<0.05). The expression of Col-ImRNA in the Niaoduqing group and Bushen Jiedu Tongluo group was decreased compared with the Model group (P<0.05). Bushen Jiedu Tongluo group and Niaoduqing group had significant difference (P<0.05).6TGF-β1mRNA:The expression of TGF-β1mRNA in the Model group, Niaoduqing group and Bushen Jiedu Tongluo group was increased compared with the normal group(P<0.05). The expression of TGF-β1mRNA in the Niaoduqing group and Bushen Jiedu Tongluo group was decreased compared with the Model group (P<0.05). Bushen Jiedu Tongluo group and Niaoduqing group had significant difference (P<0.05).7BMP-7mRNA:The expression of BMP-7mRNA in the Model group, Niaoduqing group and Bushen Jiedu Tongluo group was decreased compared with the normal group(P<0.05). The expression of BMP-7mRNA in the Niaoduqing group and Bushen Jiedu Tongluo group was increased compared with the Model group (P<0.05). Bushen Jiedu Tongluo group and Niaoduqing group had significant difference(P<0.05). Conclusion lBushen Jiedu Tongluo recipe could reduce renal function in rats with chronic renal failure.2 Bushen Jiedu Tongluo recipe could reduce the pathological damage in rats with chronic renal failure, and it was better than Niaoduqing.3 Bushen Jiedu Tongluo recipe could alleviate chronic renal failure and reduce the renal interstitial fibrosis by reducing the expression of Col-I,TGF-β1,Col-I mRNA and TGF-β1mRNA, increasing the expression of BMP-7 and BMP-7mRNA.4 The side of expression of Col-I,TGF-β1,BMP-7,Col-I mRNA,TGF-β1mRNA and BMP-7mRNA effected by Bushen Jiedu Tongluo recipe was better than its by Niaoduqing.