Effects Of Exercise On2,3,7,8-TCDD Induced Hepatotoxicity In Rats

Objective:The aim of the current study was to explore the possible effect of exercise to hepatotoxicity in2,3,7,8-TCDD induced, and provide the basis for researching the biomarkers.Methods:fifty animals were assigned randomly into five groups of ten each, including a normal control group (NC), two toxic groups (NT1,NT2) and two exercise toxic groups(ET1,ET2). The rats of toxic groups and exercise toxic groups received10μg/kg BW of2,3,7,8-TCDD, the NC group were administered with equivalent volumes of coin oil only, and exercise groups were swimming with5%weights attach to tails for30min daily. Body weights were recorded weekly, and the blood was collected for serum biochemical analysis, the livers were immediately isolated and weighted, and the lobe of each liver were kept at-80℃for detect the activity of hepatic microsomal enzymes and Proteomics analysis.Results:There were no effects on general health in all group. Significantly increased of Liver weight, Liver relative weight (P<0.01), the serum levels of aspartate aminotransferase (AST), Phospholipid (PL), Total cholesterol(TC)(P<0.01)and the activity of aryl hydrocarbon hydroxylase(AHH)(P<0.05) were observed in TCI group. Significantly decreased were observed of the serum levels of TC (P<0.01)and activites of7-ethoxycoumarin O-deethylase(ECOD)(P<0.01)in TE1group. Significantly decreased were observed of Liver relative weight (P<0.01), and significantly increased of serum levels of AST,TC(P<0.01) and activities of7-ethoxyresorufin0-deethylase (EROD), ECOD and AHH(P<0.01) in ET2group. Analysing the49points of difference by mass spectrometry, the number of positive points was24, and the number of posoitive points of difference was8. Exercise could affected the expression of liver protein, the protein up-regulated were flavin reductase and actin, while the protein down-regulated were cytochrome b5,78kDa glucose-regulated protein precursor,3alpha-Hydroxysteroid(Slash)dihydrodiol Dehydrogenase and glutathione S-transferase alpha-3. Exercise pre-adaptation could also altered the expression of liver protein. Conclusion:TCDD could induced the hepatotoxicity. Exercise could effective prevent the hepatotoxicity induced TCDD, and whether exercise pre-adaptation could prevent the hepatotoxicity induced TCDD need further research.