| This dissertation focused on an investigation of main constituents of Lonicera japo nica Thunb belonging to the genus Lonicera Linn of Caprifoliaceae and Morus australia belonging to the gesus of Morus of Moraceae. Totally54compounds were isolated and their structure were elucidated by spectroscopic and chemical methods. The bioassays of their extract and pure compounds were also carried out on various pharm cological mode-ls.The buds of Lonicera japonica was collected in Shandong province and identified by Professor Lin Ma, the Institute of Materia Medica, Chinese Academy of Medical Sci-ences&Peking Union Medical College. The barks of Morus australis was collected in Jiangxi province and identified by Professor Ce-ming Tan, the Jiujiang Botany Institute of Forest, Jiangxi province.Forty compounds were isolated from Lonicera japonica Thunb, they were identifi ed as follows:Lonijapospiroside A(l*), L-phenylalaninosecologanin B (2*), L-phenyla laninosecologanin C(3*), Dehydroprolinoylloganin A (4*), loganin (5), secologanin (6), Secoxyloganin(7), Dimethyl-secoxyloganin(8), vogeloside(9),7-epi-vogeloside(10), Sweroside(11),7-ethyl-epi-vogeloside(12),(E)-aldosecologanin (13), Loniceracyclo side(14), urceolide(15), abscisic acid(16),(1S,6R)-8-(-hydroxya bscisic acid)-β-D-glucopynaloside)(17), Limonin(18), Loniceroside A (19), Scopole tin(20), Xanth-otoxin(21), isopimpinellin(22), oroseol(23), emodin(24),1-O-β-D-glucopyranosyl-emodin(25),3-O-caffeoylquinic acid(26),4,5-di-O-caffeoyl isoquinic acid(27),3,4-di-O-caff-eoyl quinic acid(28),3,4-di-O-caffeoyl quinic acid methyl ester(29),4,5-di-O-c-affeoyl quinic acid methyl ester(30),3,4-di-O-caffeoyl quinic acid ethyl ester(31),3,5-di-O-caffeoyl quinic acid ethyl ester (32), luteolin-7-O-β-D-gluco pyranoside(33), chrysoeriol-7-O-β-D-glucopyranoside (34), Lonicerin (35),5,4’-dihydr oxy—3’,5’-dimethoxy-7-p-D-glucosyloxy-flavone (36), quercetin(37),(E)-2-Hexen yl-α-L-arabino pyranosyl-(1â†'6)-p-D-glucopyranoside (38), Benzylalcohol-O-a-L-arabi nopyranosyl-(1â†'6)-O-β-D-glucopyranoside (39), Eugenyl-β-D-xylopyranosyl-(1â†'6)-β-D-glucopyr anoside (40). Among these compounds, there were four new compounds belong to secoiri-doid with nitrogen, and12known compounds from the genus Lonicera for the first time. The structure types of the compounds included iridoid, monoterpenoid, sesquiterpenoid, triterpenoid, coumarin, anthraquinone, caferoylquinic acid, flavonoid, most of which were in glycoside.Fourteen compounds isolated from Morus Australia, were listed as follows:Benzo-kuwanon E (MA-1), Hydroxymorusin(MA-2), Dicyclokuwanon EA(MA-3), Dicyclo-kuwanon EB(MA-4), Kuwanon E(MA-5), Kuwanon U(MA-6), Kuwanon S(MA-7), Morusignin L(MA-8), Oxydihydromorusin(MA-9), Morusin(MA-10), Kuwanon C (MA-11), cyclomorusin(MA-12), mulberranol(MA-13), Hydroxymorusin(MA-14). MA-1-MA-4were four new flavonoids.The fraction D15, D30(eluting from macropore resin D101with ethanol15%,30%respectively) showed anti-flammatory activity on croton oil ear in mice. Within the compouds from Lonicera japonica Thunb, compounds2,14,30showed inhibitory activities against the release of NO in mice by Gibbs reduction method with inhibition rate of25.7%,44.6%,25.4%at10-5M, respectively. The later two showed no cytoxity but the former showed significant cytoxity on the same pharmacological mod-el. Compound15exhibited inhibitive activities against TNF-a in mouse macrophage wi-th inhibition rates of45.9%at10-5M, while compound27,32with inhibition rates about20%in the same condition. All of the compounds tested had no apoptosis activities on MCF7GFP-Bax model.Compounds tested showed no antivirus activities on VSVG-HIV model at10-5M.All the extracts and compounds from Lonicera japonica Thunb were inactive against human cancer cell lines(A549, Bel-7402, BGC-823, HCT-8and A2780) at10-5M on MTT model.Among compounds isolated from Morus Australia, only MA-2inhibited the producti-on of the lipid peroxide induced by Fe2+-Cys system in the liver microsomal, with inhibi-tory rates10%at10-5M. |
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