The Empirical And Clinical Study The Herbs Of YiQiYangXueWenYang Treatment On Constipation

Objectives:Study the mechanism of the herbs of Yi qi yang xue wen yang(YQYXWY) treatment on constipation lead by laxatives. To observe therapeutic effect of herbs of Yi qi yang xue wen yang treatment on constipation of the aged; and to evaluate its side effect and its safetyMethods:empirical study：After constipation lead by laxatives rat model was established,they are divided into6groups:model group, normal group,PPEG4000group(control group),the high dose of YQYXWY(YQYXWY-H), the medium dose of YQYXWY group(YQYXWY-M) and low dose of YQYXWY(YQYXWY-L) group.The control group rats took medicine of PPEG4000,model group and normal group took medicine of physiological saline. The other three groups took medicine of herbs YQYXWY at high dose, medium dose, low dose. They were treated by2weeks. Then the rats were sacrificed, the colonic transit fuction were measured by ink propulsion test. We excised rats’colon specimen.We applied with immunohistochemical technology to analyze the expression of VIP, NOS, SP. The expression of interstitial cells of Cajal in colon was analyzed by RT-PCR. Clinical trial:50old patients with chronic constipation were divided randomizedly to the study group30and the control group20. The study group take medicine of herbs of Yi qi yang xue wen yang, the control group take medicine of PPEG4000(Poly (ethylene glycol)4000). The course of treatment is2weeks. We recorded the change of symptom scores.All counted data was expressed in the form of χ±s, and dealed by SPSS11.5statistics software.Results:empirical study:The intestinal propusion rate in YQYXWY groups was significantly increased than that in model group (P<0.05).The herbs of YQYXWY can increased the expression of SP in colon of rat, that in YQYXWY-H group is higher than model group (p<0.05). VIP in YQYXWY-H group is lower than model group (p<0.05). The herbs of YQYXWY can degrade the level of NOS in colon tissue. The expression of NOS in the YQYXWY-H group, YQYXWY-M group and YQYXWY-L group is lower than that in model group (p<0.05). The herbs of YQYXWY can increase expression of interstitial cells of Cajal in the colon of rat; the expression of interstitial cells of Cajal in the YQYXWY-H group, YQYXWY-M group is higher than that in model group (p<0.05). Clinical study:There were no differences in the overall effect and constipation integral between the two groups (p>0.05). Great differences were found in the stool property integral and TCM symptoms integral between the two groups. No side effects such as abdominal pain, diarrhea were found in the study group.Conclusions:The herbs of YQYXWY can increase the intestinal propusion rate in the rat model constipation led by laxative. It also can increase expression of interstitial cells of Cajal, SP and degrade expression of VIP, NOS in the colon of rat model constipation led by laxative. The herbs of Yi qi yang xue wen yang is effective and safe in treatment of constipation of the age.