| Objective:To observe the effects of acupuncture on first time defecation, defecation grainnumber in12h and small intestinal propulsion rates in constipation mice; Comparing theeffect between single point groups and Acupoints combination groups for the treatmentof constipation; To discuss the differences between the meridians and acupoints, thecollaborative of acupuncture effect and the otherness between acupuncture point groups;And based on this, To observe the effects of acupuncture on express of ICC inconstipation mice.To reveal the mechanism of action in acupuncture by the interventionto constipation model mice’s small intestine movement, Provide experimental basis forthe theory of Meridians viscera.Method:①The comparison of single point groups: Choose60healthy kunming mice who’sbody quality for20-30g, divide into several groups in random: normal group, modelgroup tianshu group dachangshu group quchi group shangjuxu group, those groups ofmice are drenched with DC, the mice are breeding in single cage, squirrel cage bottomare pad with filter paper,fasting for12h and Free water before molding, the normalgroup treat normally, model group treat without acupuncture treatment, The rest groupsare treating with acupuncture, Record for the first time after the first groups of mice bygastric bowel movement time In the experiment, Collect and record within12h afterlavage defecation grain number, keep the four days’ cumulants as observed value. Therest groups are treating with acupuncture, and fast semisolid paste0.8ml each mice afterthe last treatment, Take off the cervical executed in mice after20minutes, Open,remove the small intestine quickly, strip and straight on white sheet of paper gently,measure the distance of pyloric to caecum and the distance of pyloric to the front ofblack semisolid paste, calculate small intestinal propulsion rates for pylorus to black semisolid paste front distance of pylorus to back the blind percentage of total length.Take2cm long and colon in4%neutral formaldehyde fixation, dehydration, paraffinembedding, sectioning, select the adjacent slices for immunohistochemical detection.②The comparison between single point group and acupoints combination group: Select50mice, divide into several groups in random: normal group, model group tianshugroup(both sides), dachangshu group(both sides), tianshu and dachangshu group(singleside); Select50mice, divide into several groups in random: normal group, model group,quchi group(both sides), shangjuxu group(both sides), quchi and shangjuxugroup(single sides). Exchange the contralateral on alternate days, The testing indexesand operation method are the same as above.③The comparison of acupoints combination groups: Select50mice, divide into severalgroups in random: normal group, model group, tianshu and dachangshu group(bothsides), quchi and shangjuxu group (both sides), four single acupoints group(tianshudachangshu quchi shangjuxu on one side), The testing indexes and operation method arethe same as above.④The mechanism of action of acupuncture intervention to constipation model mice’ssmall intestine movement: Select normal group, model group and four single acupointsgroup(the best curative effect), Select8slices each group, and calculate the ICC cellnumber.Results:①The comparison of single point groups: Compared with normal group, The first timedefecation in model mice were significantly prolonged (P<0.01), Waste grain numberwithin12h significantly reduce(P<0.01), The intestinal propulsion rate significantlyreduced(P<0.01); Compared with model group, each single point acupuncture group ofmice’s first time defecation significantly shorten(P<0.05). Waste grain number within12h significantly increase(P<0.05), The intestinal propulsion rate significantlyimproved P<(0.01);②The comparison between single point group and acupoints combination group: Compared with normal group, The first time defecation in model mice weresignificantly prolonged(P<0.01), Waste grain number within12h significantly reduce(P<0.05), The intestinal propulsion rate significantly reduced (P<0.01); Compared withmodel group, each single point acupuncture group of mice’s first time defecationsignificantly shorten. Waste grain number within12h significantly increase (P<0.05),The intestinal propulsion rate significantly improved (P<0.05); tianshu and dachangshugroup(single side) compared with tianshu group, dachangshu group, quchi andshangjuxu group(single sides) compared with quchi group, shangjuxu group aredifferent on first time defecation, defecation grain number in12h and small intestinalpropulsion rates(P<0.05).③The comparison of acupoints combination groups: Compared with normal group, Thefirst time defecation in model mice were significantly prolonged (P<0.01), Waste grainnumber within12h significantly reduce (P<0.01), The intestinal propulsion ratesignificantly reduced (P<0.01); Compared with model group, each single pointacupuncture group of mice’s first time defecation significantly shorten(P<0.05). Wastegrain number within12h significantly increase (P<0.05), The intestinal propulsion ratesignificantly improved(P<0.05); four single acupoints group(tianshu dachangshu quchishangjuxu on one side) compared with tianshu and dachangshu group, quchi andshangjuxu group are different on first time defecation, defecation grain number in12hand small intestinal propulsion rates(P<0.05).④The comparison of ICC cell number: Compared with normal group, Model groupmice cells was significantly reduced(P<0.01); Compared with model group,acupuncture group ICC cells was significantly increased in mice (P<0.01).Conclusion:①Needing a single point(quchi, shangjuxu, tianshu, dachangshu) on constipation isobviously improved, There was no significant difference of them.②The therapeutic effect of acupuncture tianshu and dachangshu group(on single side)is better than acupuncture tianshu group(both side) or dachangshu group (both side), The therapeutic effect of acupuncture quchi and shangjuxu group(on single side) isbetter than acupuncture quchi group(both side) or shangjuxu group (both side), Thereare synergies between the tip point.③The therapeutic effect of acupuncture four single acupoints (quchi, shangjuxu,tianshu and dachangshu) is better than acupuncture tianshu and dachangshu group orquchi and shangjuxu group,it prompts that: acupuncturing four single cavity formulatreatment effect is best.④Acupuncture treatment of chronic shows constipation may implementate through thecolon tissue expression of ICC. |
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