Effects Of Cluster Needling Of Scalp Acupuncture Combined With Rehabilitation Technique On Glial Cell Environment And Cellapoptosis In Rats After Focal Cerebral Ischemia

ObjectiveTo observe the effect of acupuncture in ischemic region of BDNF and S100β and GFAP protein and method on focal cerebral ischemia rats, and expression of BDNFmRNA, Caspase-3mRNA.MethodEstablishment of rat permanent focal cerebral ischemia model, In these standards in line with the selected240rats. Divided into5groups, Sham operation group, model group, acupuncture group, the rehabilitation group and acupuncture combined with rehabilitation group. Each group had48rats, Then each group at3,7,14.21days were divided into4groups,12rats in each group. Sham operation group, model group without any intervention, The acupuncture group were treated with head acupuncture therapy, The rehabilitation group was treated with treadmill training, Acupuncture combined with rehabilitation group was treated with head acupuncture treatment combined with treadmill training. Each time, Changes in the immunohistochemical detection of BDNF,S100β、GFAP in ischemic rats expression. point of the changes of nerve function test observation of neural function in rats, The detection of BDNF-mRNA,Caspase-3mRNA real-time fluorescent quantitative PCRResult1. The neurological function score, Each time point in each group, the neurological function score increased clearly (P<0.01); After7th days of treatment compared with the model group. The nerve function score of acupuncture group, rehabilitation group and comprehensive group decreased clearly (P<0.05). Compared with the acupuncture group and rehabilitation group, comprehensive group of neural function score was significantly lower (P<0.05). Acupuncture group and rehabilitation group was compared with similar.2Expression of BDNF in the ischemic area:After the success of the model,3days,7days,14days, each group and sham operation were statistically significant(p<0.01).7days after making models, compared with the model group, expression of BDNF was significantly reduced in acupuncture group, rehabilitation group and comprehensive group (P<0.05).14days after making models, Comprehensive group compared with the model group were significantly different(p<0.01).3The expression of S100β around ischemic cortex:on the3rd and7th day after the modeling, there were statistically difference between the sham operation group and other groups (P<0.05). On the3rd day after the modeling, to compared with the model group,S100β expression promoted in the CNSP combined with task-oriented training group and in the task-oriented training group (P<0.05). on the14th day after the modeling, there were statistically difference between the CNSP combined with task-oriented training group and model group (P<0.01). On the21St day after the modeling, there were statistically difference amongst in the CNSP combined with task-oriented training group, the task-oriented training group, the CNSP group and the model group(P<0.05).4The expression of GFAP around ischemic cortex:On the7th and14th days after the modeling, to compared with the model group, the expression of GFAP in the CNSP combined with task-oriented training group, the task-oriented training group and the CNSP group enhanced (P<0.05), and they in the CNSP combined with task-oriented training groupwere superior to the task-oriented training group and the CNSP group (P<0.05) respective.There were statistically difference amongst in the CNSP combined with task-orientedtraining group, the task-oriented training group and the CNSP group (P<0.05). On the21St day after the modeling, the expression of GFAP in the CNSP combined with task-oriented training group were superior to the others (P<0.05).5The expression of BDNFmRNA:Each time point in each group compared with the model group, BDNFmRNA expression was significantly increased (P<0.01). After7th days of treatment compared with the model group, expression of BDNFmRNA was significantly higher in acupuncture group, rehabilitation group and comprehensive group (P<0.05). Compared with the acupuncture group and rehabilitation group, Expression of BDNFmRNA was significantly higher in comprehensive group (P<0.05).Acupuncture group and rehabilitation group was compared with similar.6. The expression of Caspase-3mRNA:Each time point in each group compared with the model group, Caspase-3mRNA expression was Increased significantly (P<0.01). After7th days of treatment compared with the model group, expression of Caspase-3mRNA was significantly reduced in acupuncture group, rehabilitation group and comprehensive group (P<0.05) Compared with the acupuncture group and rehabilitation group, Expression of Caspase-3mRNA was significantly reduced in comprehensive group (P<0.05).Acupuncture group and rehabilitation group was compared with similar.Conclusion1Acupuncture and rehabilitation can promote the recovery of neural function of rats after focal cerebral ischemia.2Acupuncture and rehabilitation can promote the expression of proliferation in brain tissue after focal cerebral ischemia GFAP, S100β.3Acupuncture and rehabilitation can promote the expression of proliferation in brain tissue after focal cerebral ischemia BDNF-mRNA.4Acupuncture and rehabilitation promotes the expression of brain tissue after focal cerebral ischemia Caspase-3mRNA attenuation.5Acupuncture combined with rehabilitation to glial cells after cerebral ischemia in environmental regulation, inhibition of neuronal apoptosis pathway. This may be one of the mechanisms of acupuncture combined with rehabilitation for cerebral ischemia nerve recovery.