| ObjectiveTo observe the effects of cluster needling of acupuncture combined with treadmill training in Motor function metabolism of serum glucolipid、NF-κB-p65、TNF-α and IL-1β in serum、ischemic region of cerebral cortex and skeletal muscle NF-κB-p65、TNF-α、IL-1β protein and skeletal muscle IRS-1and PI3K expression of gene in rats with Diabetes ischemic stroke. To discuss the probable mechanism applied to improve the neurological function defects and IR in rats.MethodEstablish rat diabetes ischemic stroke model. To randomly divide192rats into six groups, which are Sham operation group, Model group, Acupuncture group, Rehabilitation group and Blank group. And then each group was divided into four subgroups in accordance with the3rd,7th,14th,21st days (n=8). No intervention was given to Sham operation group, Blank group and Model group, while the Acupuncture group was treated with head combined with body acupuncture therapy, the Rehabilitation group was treated with treadmill training, Acupuncture combined with rehabilitation group was treated with acupuncture treatment combined with treadmill training. Each time the changing of rats’ neurological function was observed. TG、TC content were evaluated by net screen test. FINS、NF-KB-p65、TNF-α、IL-1β content were evaluated by ELISA. Ischemic region of cerebral cortex and skeletal muscle NF-κB-p65、TNF-α、IL-1β were evaluated by SABC. And the Real-Time PCR staining was used to observe the expression of skeletal muscle’s IRS-1mRNA、PI3K mRNA.Results1. Scores of Balance Beam test:Compared with Sham operation group, the score of each group was significantly improved at each point (p<0.01). The3rd,7th,14th,21st day after the modeling, the score of Acupuncture combined with rehabilitation group dropped down (p<0.05). And compared with Acupuncture group and Rehabilitation group, the score of Acupuncture combined with rehabilitation group dropped down (p<0.05) on the14th and21st day after the modeling.2.Metabolism of glucolipid:①FPG:Compared with the blank group, the FPG of the remaining groups have been significantly improved on the14th and21st day after the modeling (p<0.01). The FPG of the Acupuncture combined with the rehabilitation group decreased clearly which compared with the Sham operation group and the Model group (p<0.01).And the Acupuncture combined with rehabilitation group’s FPG had been decreased compared with the Acupuncture group (p<0.05).②FINS:Compared with the blank group, the FINS of the remaining groups have been significantly improved on the14th and21st day after the modeling (p<0.01).Compared with the Sham operation group and the Model group, the FINS of the Rehabilitation group decreased clearly on the21st day after the modeling (p<0.01). The acupuncture combined with rehabilitation group’s FINS decreased clearly (p<0.01). And the FINS of acupuncture combined with rehabilitation group’s FINS had a decrease compared with the acupuncture group (p<0.05).③IAI:Compared with the blank group, the IAI of the remaining groups was significantly reduced on the14th and21st day after the modeling (p<0.01). Compared with the sham operation group, the IAI of the rehabilitation group increased clearly on the21st day after the modeling (p<0.05). The acupuncture combined with rehabilitation group’s IAI decreased clearly on the14th and21st day after the modeling (p<0.01). And the IAI of acupuncture group’s IAI was been decreased compared with the model group (p<0.05). The.acupuncture combined with rehabilitation group’s IAI decreased clearly on the14th and21st day after the modeling (p<0.01). Compared with the acupuncture group, the IAI of the acupuncture combined with rehabilitation group had a decrease on the14th and21st days after the modeling (p<0.05).④TG:Compared with the blank group, the TG of the remaining groups was significantly reduced on the14th and21st day after the modeling (p<0.01). Compared with the sham operation group and the model group, the TG of the acupuncture combined with rehabilitation group dropped down on the21st day after the modeling (p<0.05).⑤TC: Compared with the blank group, the TC of the remaining groups was significantly decreased on the14th and21st day after the modeling (p<0.01). Compared with the sham operation group and the model group, the TC of the acupuncture combined with rehabilitation group dropped down on the14th and21st day after the modeling (p<0.05). Compared with the model group, the TC of the acupuncture group dropped down on the21st days after the modeling.3. The content of NF-κB-p65、TNF-α、IL-1β in serum:Compared with the blank group and the sham operation group, the NF-κB-p65、TNF-α IL-1β content in serum of the remaining groups was significantly decreased on each point after the modeling (p<0.01). Compared with the model group,the content of the NF-KB-p65、TNF-α、IL-1β in serum of the which acupuncture combined with rehabilitation group have been decreased (p<0.01). Compared with the acupuncture combined with rehabilitation group, the NF-KB-p65contents of the acupuncture group decreased (p<0.01) and the rehabilitation group (p<0.05) are not clearer than acupuncture combined with rehabilitation group on the7th day after the modeling. The content’s decrease of the acupuncture group is not clearer than acupuncture combined with rehabilitation group on the14th and21st day after the modeling. Compared with the model group, the TNF-α content of the acupuncture group,the rehabilitation group、 the acupuncture combined with rehabilitation group have been decreased (p<0.01) on the7th and14st day after the modeling, the content of the rehabilitation group(p<0.05) and the acupuncture combined with rehabilitation group(p<0.01) decreased on the21th day after the modeling。Compared with the acupuncture combined with rehabilitation group, The content’s decrease of the acupuncture group and the rehabilitation group is not clearer than it on the7th and14st day after the modeling(p<0.05). Compared with the model group, the IL-1β content of the acupuncture group have been decreased (p<0.05) on the3th and7st day after the modeling; the rehabilitation group decreased (p<0.05) on the3th and14st day; the acupuncture combined with rehabilitation group decreased (p<0.01) on every day. Compared with the acupuncture group, the acupuncture combined with rehabilitation group decreased (p<0.05)on the21st day.4. The expression of NF-κB-p65、TNF-α、IL-1β around ischemic cortex:Compared with the sham operation group and the blank group, there was a significant increase of NF-κB-p65、TNF-α、IL-1β expression in the remaining groups on each point (P<0.01). Compared with blank group, the sham group have been increased (P<0.01). Compared with model group, the NF-KB-p65expression reduced in acupuncture combined with rehabilitation group on every day after modeling (p<0.01). Compared with acupuncture group and rehabilitation group, expression reduced in acupuncture combined with rehabilitation group on3rd and2st day after modeling (p<0.05). Compared with model group, the TNF-α expression reduced in acupuncture combined with rehabilitation group on every day after modeling (p<0.01). Compared with acupuncture group, expression reduced in acupuncture combined with rehabilitation group on7rd (p<0.01) and3rd,21st(p<0.05) day after modeling, Compared with rehabilitation group, expression reduced in acupuncture combined with rehabilitation group on14st (p<0.05) day after modeling。Compared with model group, the IL-1β expression reduced in acupuncture combined with rehabilitation group on3rd、7th、14th day (p<0.01) and21rd day(p<0.05). Compared with acupuncture group and rehabilitation group, expression reduced in acupuncture combined with rehabilitation group on3rd,7th (p<0.05).5.The expression of skeletal muscle NF-κB-p65/TNF-α IL-1β:On the21st day after modeling, compared with the blank group,there was a significant decrease of NF-κB-p65、 TNF-α、IL-1β expression in the remaining groups (P<0.01), compared with the sham operation group and the blank group,there was a significant increase of NF-κB-p65、TNF-α IL-1β expression in the remaining groups expect of the blank group (P<0.01), compared with acupuncture group, the expression had a significant reduce in rehabilitation group (p<0.05), the expression reduced clearly in acupuncture combined with rehabilitation group (p<0.01), compared with rehabilitation group, the expression reduced in acupuncture combined with rehabilitation group (p<0.05).6. The expression of skeletal muscle IRS-1mRNA:On the21st day after modeling, there was the skeletal muscle IRS-1mRNA expression in every group there was a significant decrease of IRS-1mRNA expression in blank group than any others (P<0.01), compared with the sham operation group and the blank group,there was a significant increase of IRS-1mRNA expression in the remaining groups expect of the blank group (P<0.01), compared with acupuncture group, the expression decreased in rehabilitation group (p<0.05),the expression decreased more clearly in acupuncture combined with rehabilitation group (p<0.01), compared with rehabilitation group, the expression decreased in acupuncture combined with rehabilitation group (p<0.05).7. The expression of skeletal muscle PI3KmRNA:On the21st day after modeling, there was the skeletal muscle PI3KmRNA expression in every group. There was a significant decrease of PI3KmRNA expression in blank group than any others (P<0.01), compared with the sham operation group and the blank group, there was a significant decrease of PI3KmRNA expression in the remaining groups expect of the blank group (P<0.01). Compared with rehabilitation group and acupuncture group, the expression decreased in acupuncture combined with rehabilitation group (p<0.05). Conclusion1. Acupuncture and rehabilitation can promote the recovery of neural function of rats after diabetes ischemic stroke, and its effect is superior to the rehabilitation or acupuncture.2. Acupuncture and rehabilitation can demote the glucolipid metabolism of rats after diabetes ischemic stroke, making insulin more sensitive, and reverse metabolize abnormal, to improve diseased organism status.3. Acupuncture and rehabilitation can demote the NF-κB-p65、TNF-α and IL-1β in brain serum and cerebral ischemic region of rats after diabetes ischemic stroke, and then demote inflammation of ischemic region of cerebral issue and protect the brain.4. Acupuncture and rehabilitation can demote the expression of skeletal muscle NF-κB-p65、TNF-α、IL-1β protein in cerebral ischemic region of rats with diabetes ischemic stroke, to help relieve inflammation, making insulin in skeletal muscle more sensitive and demote insulin resistance.5. Acupuncture and rehabilitation can promote the expression of skeletal muscle IRS-1mRNA、PI3KmRNA protein in cerebral ischemic region of rats with diabetes ischemic stroke. Improvements demote the insulin resistance of skeletal muscle probability by IRS-1/PI3K signal transduction pathway. |
PDF Full Text Request