The Biomimetic Construction Of Film On Titanium Implant And The Research Of Its Biological Behavior

As hard tissue implants, pure titanium is widely used due to its advantages inbiocompatibility, mechanical properties, corrosion resistance, and light weight.However, the application of pure titanium is limited in clinic because of its lowbiological property, which leads to the poor interface of the implant-bone tissue. Inthis research, the clickable ECM-MAO film, which could improve the biologicalproperties of the pure titanium hard tissue implant, was biomimetic constructed onthe implant surface by using micro-arc oxidation, decellularization and click reaction.In the ECM-MAO film, the MAO film is mainly formed by anatase, rutile andapatite. This MAO film could load the allen sodium phosphate (AL) and control therelease of AL up to50h. On the other hand, it could also load the antimicrobial drugPAMAM Dendrimer stably up to30days in PBS solution. The MAO film withPAMAM Dendrimer could exhibit the antimicrobial activity to bacteria P. aeruginosaand S. Aureus while keeping excellent biocompatibility and bioactivity to HBMSC(Human Bone Mesenchymal Stem Cells). The ECM film in the ECM-MAO film isconstructed from the decellularized film model. The decellularized model is form bythe extracellular matrix (ECM) secreted by HUVEC (Human Umbilical VeinEndothelial Cells), which could improve the adhesion, proliferation and angiogenesisof HUVEC in vitro compared to the control groups. The ECM-MAO film which isbiomimetic constructed from this model and formed by ECM secreted by HUVEC orHBMSC shows better biological behavior than MAO film. It could improveHBMSC’s proliferation and expression of Col-I and OPN in vitro.For ECM-MAO film, the high biocompatibility of the chemistry method is required toprotect the bioactive molecular in ECM while integrating bioactive molecular on it.Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) is one kind of the click reactions withhigh biocompatibility. After researched the effect of reactive condition on the reactive rateand biocompatibility, we use CuAAC to stain the live cell and bacteria with dye BN, CN andthe new dye BCN designed by us. After reaction, the cell and bacteria could exhibitfluorescence while keeping alive. At the same time, compared to BN and CN, the BCN could exhibit two kinds of fluorescence. This click reaction supports a kind of method to integratebioactive molecular on the clickable ECM-MAO film.Finally, we import the alkyl group into the ECM film to construct the clickable ECMmodel. After integrated QK with click reaction, the model could improve the angiogenesis ofHUVEC evidently. The clickable ECM-MAO biomimetic film is biomimetic constructedfrom this model, which could show better biological behavior for improving the expression ofosteogenic gene of HBMSC after integrated with QK by click reaction. In addition, comparedthe film integrated QK peptide in air, the film integrated QK peptide in nitrogen has bettereffect on improving the expression of osteogenic gene of HBMSC at the first day.