Studies On Constituents Of Two Kinds Of Traditional Chinese Medicine In Lingnan Area

There are many species of medicinal plant resources in Lingnan area, which is one of the main medicinal material sources in China. Many famous-region drugs have been widely used for thousands of years. This thesis focused on the isolation and structure elucidation of constituents from Rabdosia lophanthoides var. gerardiana and Morinda officinalis which are two kinds of genuine medicine materials of Lingnan area.Rabdosia lophanthoides (Buch. Ham. ex. D.Don) Hara. var. gerardiana (Benth.) Hara.] belonging to the genera Rabdosia of the family Labiatae, is one source of Chinese medicine "Xihuangcao".Medicine and pharmacology have been proved that Xihuangcao has certain anti-cancer, antibiosis, anti-inflammatory and liver protective effects.We confirmed the anti-inflammatory parts according to the vitro experiment and 25 compounds were isolated from the whole herbs of R. lophanthoides var. gerardiana by bioassay-guided fractionation. Their structures were established as rabdosin D (1),(+) 15-hydroxy-salvinolone (2), rabdosiacoside A (3), (-)-sesaminone-rutinoside (4), (+)-episesaminone-rutinoside (5), rabdosiacoside B (6), rabdosiacoside C (7),16-acetoxy horminone (8),α-hinokiol (9), isodoforrestin (10), rosmarinic acid (11),methyl rosmarinate (12), perovskoate (13), (+)-1-hydroxypinoresinol-1-O-β-D-glucoside (14), (-)-pinocembrin(15), quercetin(16), kaempferol (17), rutin (18), scopoletin (19), 6,7-dihydroxycoumarin (20),3,4-dihydroxy-benzoic acid (21),caffeic acid (22), caffeic acid ammonium (23), oleanolic acid (24) andβ-sitosterol (25) on the basis of physical chemical properties and spectroscopic analysis. Compounds 1-7 were new ones, while 9,15,19 were isolated from the genus of Rabdosia for the first time.According to the results of the pharmacological screen, several diterpenes, phenylpropanoids were found to be able to inhibit the release of inflammatory mediators (NO、TNF-α、IL-6) and key enzyme (COX-2), while the main constituent could inhibit the activity of 15-LO.Morinda officinalis How. belongs to the genera Morinda of the family Rubiaceae.It has been used to reinforce kidney, cure impotence, be good at spermatogeny, womb cool barrenness, irregular menstruation and dispel rheumatism in traditional Chinese medicine.A phytochemical investigation on root bark of M. officinalis resulted in the isolation of 28 compounds.Their structures were established as morindasin A (26),asperulosidic acid (27), deacetyl asperulosidic acid (28),monotropein (29), monotropein methyl ester (30),6α-hydroxyadoxoside (31), 6a-hydroxy-7-epiloganin (32),pedicularis-lactone (33), (+)-camphor (34), (1S,2R,4S)-borneol-O-β-D-glucopyranoside (35), rubiasin A (36), rubiasin B (37), 2-methyl-anthraquinone (38),2-hydroxy-l-methoxy-anthraquinone (39), 3-hydroxy-l-methoxy-2-methyl-anthraquinone (40), 1,3-dihydroxy-2-methyl-anthraquinone (41), 1,3-dihydroxy-2-methoxy-anthraquinone (42),2-hydroxymethyl-anthraquinone (43), 3-hydroxy-1,2-dimethoxy-anthraquinone (44),1,8-dihydroxy-3-methoxy-6-methyl-anthraquinone (45),2-phenylethyl-O-β-D-glucopyranoside (46), sec-butyl-O-β-D-glucopyranoside (47), 3,4-dihydroxyphenylethanol (48),3-(4-hydroxyphenyl)-1,2-propandiol (49),ferulic acid (50), ursolic acid (51),butane diacid (52) andβ-sitosterol (53).Among them, compound 26 was a new one, compound 32 was a new natural product, while 30,33-37,46-50 were isolated from the genus of Morinda for the first time.