Studies On The Chemical Constituents From The Roots Of Morinda Officinalis How.

Objective:Morinda officinalis How., one of the "top four south authentic traditional Chinese medicines", is a commonly used Chinese herb distributed in the south of China, and its roots are widely used for the treatment of sexual impotence, spermatorrhea, irregular menstruation and female infertility. Previous phytochemical researches have revealed that anthraquinones, iridoids, saccharides are the main constituents of this specie. The pharmacology activities of immunomodulation, anti-aging, anticancer, antidepressant, fertility and oestrogenicity had been reported in modern pharmacology. TCM theory holds that senescence is the kidney deficiency the first, then the spleen deficiency, and the Qi and blood deficiency the last, therefore, to tonify kidney, invigorate spleen and benefit vital energy are the basic means of anti-aging. Morinda officinalis How. has a concrete manifestation of anti-aging effects on the its replenishing the kidney and strengthening the bones and muscles activities. To investigate the antiaging and antifatigue active constituents in this plant, the study were performed.Methods:several methods have been used in the processes of separation and purification, including polyamide chromatography column (CC), Pre-HPLC, Sephadex LH-20 CC, and so on. their structures were identified on the basis of physical-chemical prorerties and spectral evidences(UV,IR,1H-NMR,13C-NMR,2D-NMR,MS).Results:As a result, nine compounds have been isolated, including four anthraquinones, three terpenoids, one coumarin and one ether. their structures were identified as:2-hydroxy-1-methoxyanthraquinone(1),3-hydroxy-1, 2-dimethoxyanthraquinone(2),2-hydroxy-3-methylanthraquinone(3), 2-carbomethoxyanthraquinone(4), isofraxidin(5), di-(2-ethylhexyl)phthalate(6), ursolic acid(7),2,5-bornanediol(8), ningpogenin(9).Conclusion:compound 4,5,6,8,9 was obtained from the genus of Morinda for the first time. The studies further enrich and improve the chemical constituents of Morinda officinalis How., provide new chemical substances for the basis study of Morinda officinalis How., provide a scientific basis for quality control and rational drug use of Morinda officinalis How.