Effects Of Four-week Hypoxic Training On Nrf2 Knockout Mice Skeletal Muscle Antioxidant

Hypoxic Training has been widely used to improve athletic ability of the athletes training methods. It tries to stimulate by hypoxia and exercise the dual factors, so that the body be a strong stress response, to maximize the mobilization of the body’s potential to produce a series of physiological and biochemical reactions helps to improve athletic ability, in order to improve exercise capacity purpose. However, the hypoxic training on skeletal muscle antioxidant molecular mechanism is not very clear at present, Nrf2- mediated hypoxic training on skeletal muscle antioxidant effects have not been reported. Therefore, this study by Nrf2 knockout and wild type mice for a period of four weeks hypoxic exercise training, the mice were observed in skeletal muscle of ROS, SOD CAT antioxidant enzymes and protein expression changes of GSH / GSSG ratio, and effect of exercise capacity, so as to provide a theoretical basis for the training effect mechanism of hypoxic training.Experiment Nrf2 knockout(KO) and wild(WT) mice were randomly divided into two groups: normoxic and hypoxic quiet group training group, 10 in each group. Normoxic resting groups: Rats in 61.72, a normal life in a cage, without applying exercise load; hypoxic training group: mice were exposed for 8 hours at hypoxic environment(11.2% oxygen concentration) in a day, and in the hypoxic environment gradient of 0, velocity of 12 m / min treadmill exercise one hour, six days a week for four consecutive weeks. After the last 48 hours of rest hypoxic training, exercise capacity measured. Then 48 hours, killed by cervical dislocation, take the mouse skeletal muscle, quickly frozen in liquid nitrogen, and then frozen at-80 degrees. Determination of skeletal muscle ROS, Nrf2 m RNA, SOD1, SOD2 protein expression and CAT, GSH / GSSG ratio and exhaustive exercise run away.The results showed that(1) wild-type mice and Nrf2 knock ROS in skeletal muscle were significantly increased after four weeks except rat hypoxia training;(2) quiet Nrf2 knockout mice Nrf2 m RNA, SOD2 and CAT protein expression and exercise capacity was significantly lower than wild-type mice;(3) four weeks hypoxic training, compared with the wild-type mice, Nrf2 knock was a significant decrease Deratting GSH / GSSG ratio significantly decreased and athletic ability.Conclusion: hypoxia training can improve the oxidation ability of mice. In the absence of Nrf2, there was no significant improvement in hypoxia training.