| The flavonoids of peony testa extracts has very high nutritional and medicinal value for a variety of nutrients and biological active substances.The extraction of flavonoids from peony testa,may shift the waste materialof value, improve the utilization value,and take on development of new direction.The author first studied the extraction technique of homogenate and enzyme assistant method to withdraw the flavonoid from peony testa, and studied the relationship between the parameters and the extraction rate,determined the optimum process parameters of extracting total flavonoids for enzyme assisted homogenate.Optimum conditions for the adsorption and desorption of macroporous resin, tested the in vitro antioxidant activity of flavonoids using a variety of methods, and carried on the related mechanism of the body antioxidant and research of the anti-fatigue.The main research contents are as follows:1.Conditions for the extraction rate were studies under homogenate and enzyme assistant method, the resμLts for two above condition are compared.Under the optimum homogenate conditions, a flavonoids concentration couLd be over49.82mg/g.Screening enzyme concentration and types, as well as treatment conditions,the pectinase is better than cellμLase and mixed enzyme,optimum concentration is0.05mg/mL, the maximum flavonoids concentration74.66mg/g,33percent over the flavonoids content under homogenate. The content of luteolin under enzyme-assisted is relatively high, respectively3.2mg/g, was four times under simple homogenate that was0.75mg/g.2.The macroporous resin purification of the extract were preliminarily discussed in suitable purification condition.The adsorption performance and desorption conditions of seven macroporous resin for peony testa extracts flavonoids was researched,the optimum parameters of adsorption and desorption were procured.The resμLts showed that D101was the optimum kind of macroporous resin among seven macroporous resins under the experimental condition.The optimum conditions of desorption by D101were as follows:the concentration was4mg/mL,the proper pH was6,the flow rate was1.0mL/min,80%ethanol was the eluent solvent,the eluent rate was1.2mL/min.The content of purity flavonoids and luteolin was46.66%and2.08%under the conditions mentioned above. The content of flavonoids and luteolin has been raised by6times3.In order to study on antioxidation of peony testa flavonoids and luteolin, this research through the antioxidant experiment in vitro, the total reducing capacity of peony testa flavonoids, DPPH and ABTS free radical scavenging ability and the reduction ability of Fe3+were determined, and compared them with BHT.The assessment system of the in vitro antioxidant capacity of experimental resμLts show, the total reduction ability of peony testa flavonoids and luteolin, and for DPPH and ABTS free radical scavenging ability, and the reduction ability of Fe3+were stronger than BHT, although peony testa flavonoids was inferior to luteolin, but they were a good natural antioxidant. The antioxidation of peony testa flavonoids is closely related to the content of luteolin4.The antioxidant effect in vivo of peony testa flavonoids were studied.Experimental resμLts show that high-dose group, middle-dose group and the vitamin E of the peony testa flavonoids compared with the the blank group can significantly improve the activity of SOD, CAT and GSH-PX in mice serum, liver and kidney tissue of.The high-dose group and mid-dose group has the ability to resist membrane lipid peroxidation and free radical scavenging activity in the serum, liver and kidney tissue.Differences in content of MDA and activity of SOD, CAT and GSH-PX in liver tissue was not significant compared with vitamin E, illustrated the ability of high dose group in the protection of membrane lipid peroxidation and scavenge free radical was equal to vitamin E group, further indicated that the peony testa flavonoids have antioxidant activity in vivo.5.According to the mechanism of the fatigue, from on macroscopic, through the exhaustive swimming experiment that the high dose group and middle dose group of peony testa extract can significantly prolong the swimming time of mice. Compared with control group, the exhaustive swimming time, high dose group increased87.5%, middle dose group increased52.5%, octacosanol increased40%. From the detection of various biochemical, the high dose group of peony testa flavonoids extract coμLd significantly reduce the serum urea nitrogen (BUN) and blood lactate acid (BLA) content and increase liver glycogen(LG) and muscle glycogen (MG) reserves after mouse movement.So the high dose of peony testa extract has anti-fatigue effect. |
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