Separation And Antibacterial Activity Research Of The Extract From Oil Peony Seed

Peony seed oil is rich in unsaturated fatty acid and linolenic acid,which is a high nutritional healthy edible woody oil.Peony seed polyphenol is a natural antioxidant and antibacterial agent,which has anti-inflammatory,antibacterial,antitumor and treatment of cardiovascular diseases,such as biological activity.There is a lack of system study of peony seed oil and seed shell polyphenol refining separation and application.For the peony seed oil and seed shell resource reuse,this paper will carry out different oil production of peony seed oil composition and physicochemical properties,using molecular distillation refining to study its light fraction and heavy fractions of peony seed oil chemical composition change.At the same time,carry out oil peony seed shell polyphenol extraction,separation,purification,identification,antioxidant and antibacterial activity,for the comprehensive development of resources of oil peony seed use providing theoretical basis and technical support.The research conclusions are as follows:By gas chromatography and mass spectrometry(GC-MS)technology,study the physico-chemical properties of peony seed oil obtained by squeezing method and leaching method.Results: the acid value and saponification value of the oil pressing method below the oil leaching method,but the peroxide value is higher than the leaching method;pressing peony seed oil contains linoleic acid(52.54 %),linolenic acid(19.62 %),palmitic acid(13.39 %)and stearic acid(5.13 %),leaching peony seed oil contains linoleic acid(49.61 %),linolenic acid(24.62 %),palmitic acid(13.23%)and stearic acid(5.36 %).Compare the differences of pressure oil by refined using molecular distillation technology.Molecular distillation refined peony seed oil squeezing method,the relative content of linoleic acid was reduced from51.85 % to 24.81%,and palmitic acid content was from 13.35% to 8.83%.But the relative content of linolenic acid in alpha was increased from 19% to 61.98%.Research the process extraction of oil peony seed shell polyphenol.Studied the effects of extraction temperature,time,ethanol concentration and liquid ratio under single factor,and combined the combination of response surface experiments to obtain the optimum extraction process.The results showed that: the optimum extraction process of ultrasound assisted extraction: 70 % alcohol concentration,solid-liquid ratio 25:1,extraction temperature of 60 ?,extraction time of 80 min,and the polyphenol rate was 5.75%.The contents of oil peony seed shell polyphenols,flavonoids,polysaccharides and protein were respectively 51.23,64.35,76.21 and 46.18mg/g.Separation and purification of oil peony seed shell polyphenols by macroporous adsorption resin,grading extraction,column chromatography,the results showed that the optimum macroporous resin purification process was X-5 resin(sample flow rate 2m L/min,elution rate 2mL/min).The purity of the oil peony seed shell polyphenols was increased from6.16% to 23.1%.The rate of polyphenol content and grading of ethyl acetate extraction were the highest parts which were respectively 69.42 % and 34.88 mg/g.By HPLC analysis of polyphenol,compounds might contain rutin,resveratrol,cinnamic acid and quercetin type composition of polyphenolic compounds in the form of the oil peony seed shell extract(F0);the extracts of ethyl acetate(F2)might contain resveratrol and cinnamic acid type composition of compounds;n-butanol extract(F3)might contain catechin,paeonol,rutin and quercetin type composition of compounds;the extracts of water(F4)might contain catechin,rutin and cinnamic acid components.The compound was obtained from ethyl acetate parts extract polarity after column chromatography,and was trans resveratrol through structure identification.Research on the antioxidant and antibacterial activities of oil peony seed shell polyphenol.The results showed that the ethyl acetate extract(F2)had better total antioxidant capacity and free radical scavenging ability of DPPH.The total antioxidant capacity was1 250.08 mg/g(BHT 226.00 mg/g),and the half inhibitory concentration was 54.25mg/L(BHT 3.43mg/L),scavenging free radical ability of DPPH from strong to weak: F4>F3>BHT>F2>F0;staphylococcus epidermidis minimum inhibitory concentration of ethyl acetate extracts(F2)was 5.2mg/mL.