Selection And Application Of Microorganism For Scouring Of Flax Roving

Flax(binomial name:Linum usitatissimum) has been a source of high quality fiber for thousands of years. It can be widely used for spinning, weaving, high-performance composite materials and other fields. In processing of flax yarn, traditionally, a high-temperature alkali method was used in flax roving degumming and bleaching. This method both consumes a lot of energy and produces chemical pollution emissions. Based on the theory of modem microbial engineering, the use of microorganism fermentation or purified enzyme is used in degumming of flax roving as an alternative to traditional chemical method.Currently, degumming of ramie has been used in an industrial scale and any other bio-methods used in bast fiber are relatively immature. In this study, microbiology and degumming methods were both researched. For microbiology, microorganism were selected using molecular biology and physiological and biochemical tests. Physiological and biochemical changes in the degumming methods were studied in the deep liquid fermentation conditions. Both microbiology and degumming method research were helpful to build efficient degumming system.Ten strains of marine bacteria were isolated from the enrichment and purified.Since polygalacturonase and xylanasc arc useful in scouring and high cellulase activities damage fiber quality, high polygalacturonase and xylanase and low cellulase activities were chosen as the standard of bacteria scouring. Physiological and biochemical analysis and molecular biology methods were used to identify. Further analysis showed that D3, D4, D8and D10for retting flax roving were better than product pectinase. For further studies, another four strains of marine fungus were also and identified. Determination of fungal fermentation enzyme activity pended further study.Flax roving degumming process was shown:bacterial seedâ†'seed fermentationâ†'bacterial fermentationâ†'degummingâ†'post-processing.D3and D4two strains screened from the marine environment were analyzed. Polyglaeturonase were taken as the enzyme activities. Tenacity and fineness were both considered as properties of fibers(flax bundles) and they were normalized to standardize then given the same weight to define a textile evaluation value as a nondimensional value to measure property of fibers. High temperature(35-40℃) and middle timc(12hours) made good production of polygalcturonase which is the key in scouring and keeping good fiber quality. But it still needs more alkaline for further scouring considering traditionally way is alkaline scouring. Goal programming was used to compute the estimated goal of maximum textile evaluation value. The results of computing indicates that maximum textile evaluation value was at coded factor(01.6820), which is initial pH of9, temperature of40℃, scouring time of12hours. The adequacy of the predicted model was examined by additional independent experiments at the suggested optimal synthesis conditions that flax rovings were scoured to verification that tenacity and fineness was22.78cN/tex and1165Nm respectively and SEM figure also supported the point.Enzyme in degumming was explored. Further analysis revealed that the level of bacteria flask concentration and enzyme activity peaked earlier than the level of fermentation, under the conditions described in the sample fermented liquid level reaches the level unglued faster rate. Activity curve results illustrate the activity of bacteria fermentation broth presence of several peaks, indicating that bacteria in the fermentation broth degumming process can be repeatedly used In the optimal fermentation conditions, the use of ammonium sulfate salting out method of fractional precipitation mainly flax degumming of fermented liquid enzyme, determine the relative enzyme activity get various enzymes ammonium sulfate saturation range, a large number of salting out, and in accordance with the commercial enzyme.Enlarge experiment were carried on on the bias. Test in Zhejiang Golden Eagle Group Co. Showed that the total program time was shorter than traditional chemical degumming and also chemical pollution and energy consumption was low. This shows degumming is completely replace traditional chemical degumming methods. Results of test in Textile Group Co., Ltd. in Hubei showed that the biological degumming solution can also be used to replace traditional chemical degumming, while the chemical degumming before inactivation of biological degumming process route is more appropriate for the future design of biological degumming enlarge technology program. Chemical supplement was favorable for biological degumming and3hours was enough to meet production requirements.In conclusion,based on the screening or through genetic engineering to build higher activity with the secretion of enzyme degumming strains selected to be more conducive degumming degumming auxiliaries and further optimize the process parameters, flax roving biological degumming approach is bound to be great progress, and ultimately used in the factory.