Development Of New Biological Dosimeter Using Surface Plasmon Resonance

The wide application of nuclear technology in the field of military and civilianinevitably accompanies by the nuclear radiation accidents. The information of absorbeddose is the foundation of triage of human beings in the accidental area as well as thekey of correct diagnosis and treatment for the suspected viticms. Therefore, theacquisition of this information is the basis for effective processing of the nuclearradiation accidents. A biological dosimeter is a detectable variation of a biologicalparameter after ionizing radiation, which is used for quantification of the absorbeddose in an exposed individual.It’ s characteristics is loyal,reusable andprognosis.Biological dose assessment can help develop a treatment strategy for theradiation victim a short time after a radiation catastrophe.The research is to develophypoxanthine guanine phosphoriobsyl transferase (HPRT), DNA breaks and ceramidebiological dosimetries, respectively. These three kinds of biological dosimeters areall based on the surface plasma resonance biosensor technology which is high sensitivity,label-free and easy to detect in the scene of the accident.In the research of establishment of HPRT biodosimetry, the SPR biosensor with6-thioguaine as a probe and the anti-HPRT antibody as a signal enhancement was firstlyused to detect HPRT. The lowest detection limit achieves at2.1ng/ml. This detectionstrategy was then used to detect HPRT in lymphocytes extracted from mice irradiatedwith gamma doses range from0.01to10Gy. There is a linear relationship between thecorresponding SPR singanl and gamma dose.The dose estimation range based on this linearrelationship is0.05～10Gy. Finally, the linear relationship was used to reconstructthe absorbed dose of mice. The reconstructed doses are similar to the “double-blinded”dose.In the research of establishment of DNA breaks biodosimetry, the SPR biosensor withalkaline phosphase as the probe was firstly used to determinate DNA breaks of calf thymusinduced by gamma dose. This detection strategy was then used to determinate DNA breaksof lymphocytes irradiated with different gamma doses (0.01～7Gy). There is anexponential relationship between the corresponding SPR singanl and gamma dose.The doseestimation range based on this expinential relationship is0.02～7Gy. Finally, theexponential relationship was used to reconstruct the absorbed dose of mice. The reconstructed doses are similar to the “double-blinded” dose.In the research of establishment of ceramide biodosimetry, the detection ofceramide in urine of mice using the SPR biosensor with the anti-ceramide monoclonalantibody15B4as the probe and anti-ceramide monoclonal antibody NHCER-2as the signalenhancement was firstly optimized.The optimized method was then used to detect ceramideof urine collected from irradiated mice (0.5～7Gy).There is a linear quandraticrelationship between the corresponding SPR signal and gamma dose. The dose estimationrange based on this linear quandratic relationship is1～7Gy. Finally, the linearquandratic relationship was used to reconstruct the absorbed dose of mice. Thereconstructed doses are lower than the “double-blinded” dose.In this study, HPRT and DNA breaks biodosimetries based on the SPR technology aresensitive, simply and rapid.Both of dose estimation range and estimation accuracy aresuperior to their traditional dosimeters.Comparison of performance of these three newlydeveloped biodosimeters, the dose estimation range and estimation accuracy of HPRTdosimeter is the best.