| ObjectiveTo observe effect of Danshen injections on Acetylcholine transferase, Glial cell line-derived neurotrophic factor, synaptophysin, synapsinI and Synaptic adhesion proteins I in the gray matter of rat after acute spinal cord injury. To deduce the mechanism about the effect of Salvia miltiorrhiza on acute spinal cord injury.Methods1.Grouping and making rat models with acute spinal cord injury:144Male SD rats were randomly divided into observation group, control group and SCI group (48in each group) Spinal cords of rats in these groups were injured with Modified Allen method. Other spinal cords of48rats in the sham group were not injured in addition to the same surgical procedure as that in SCI group.2. Delivery method(1) Observation group:Salvia miltiorrhiza injection was injected into rats’abdominal cavity in one hour (Once a day, and respectively injected for one time, three times, seven times, fourteen times according to different groups)(2) Control group:Used large dose of shock therapy, methylprednisolone was injected into rats’ abdominal cavity with30mg/kg first, and then respectively injected into rats’ abdominal cavity in0.5hour, lhour,2hour and4hour with4.5*23/4mg/kg.3.Indexes detection(1)Behavioral score:Assessing the spinal cord function with inclined plate method improved by Rivlin (2) Histopathology:With optical microscope, observating the change in cell structure in1days、3days、7days and14days after acute spinal injury.(3)hybridization in situ:Including acetylcholine transferase, glial cell line-derived neurotrophic factor, synaptophysin, synapsinI and synaptic adhesion proteinsl.4. Statistical analysis:The data will be calculated with SPSS11.0.Results1. Behavioral score:The critical angle of inclined plane test was significantly reduced in observation group and control group on the first day, the third day after modeling. There was not significant difference between observation group and control group(P>0.05). There was a slight rebound in critical angle on the seventh day and the fourteenth day after modeling. The critical angle in control group was greater than that of the observation group on the seventh day(P<0.05). There was not significant difference between observation group and control group in the critical angle of inclined plane test on the fourteenth day(P>0.05).2. Histopathology:Bleeding, edema, thin nissl bodies in cytoplasm were found in damage zone of gray matter of observation group and control group on the first day after modeling, with part of denatured and necrotic neurons. Bleeding and edema were reduced in observation group on the third day, with slightly increased neuronal necrosis. Bleeding and edema were reduced in control group on the third day, with increased neuronal necrosis. Bleeding and edema were significantly reduced in damage zone on the seventh day and the fourteenth day, with thick nissl bodies in cytoplasm.4. Glial cell line-derived neurotrophic factor(GDNF):The absorbance value of GDNF positive products in observation group was less than that of control group(P<0.05), but more than that of SCI group(P<0.01)on the first day after modeling. It was less than that of control group(P<0.05), but more than that of SCI group(P<0.05)on the third day. It was not significant difference from control group(P>0.05), but more than that of SCI group(P<0.01)on the seventh day and the fourteenth day(P>0.05).3. Acetylcholine-converting enzyme(ChAT):The absorbance value of ChAT positive products in observation group was less than that of control group(P<0.01), but not significant difference from SCI group(P>0.05)on the first day after modeling. It was less than that of control group(P<0.05), but more than that of SCI group(P<0.01)on the third day and the seventh day. It was more than that of SCI group(P<0.01), but was not significant difference from control group(P>0.05)on the fourteenth day.5. synaptophysin:The absorbance value of synaptophysin positive products in observation group was not significant difference compared with control group and SCI group on the first day and the third day after modeling(P>0.05). It was more than that of SCI group(P<0.01), but was not significant difference compared with control group(P>0.05)on the seventh day and the fourteenth day.6. synapsin I:The absorbance value of synapsin I positive products in observation group was not significant difference compared with control group and SCI group on the first day and the third day after modeling(P>0.05). It was more than that of SCI group(P<0.01),but was not significant difference compared with control group(P>0.05)on the seventh day and the fourteenth day.7. Synaptic adhesion proteins I (syt I):The absorbance value of syt I positive products in observation group was not significant difference compared with control group and SCI group on the first day after modeling(P>0.05). It was less than that of control group(P<0.05), but more than that of SCI group(P<0.01)on the third day. It was more than that of SCI group(P<0.01), but was not significant difference compared with control group(P>0.05)on the seventh day and the fourteenth day.Conclusion1. Animal model in this experimentation was repeatable, reliable and controllable.2. Protection of Salvia for spinal cord gray matter after acute spinal cord injurySalvia could increase ChAT, GDNF, synaptophysin, synapsinI and sytI in spinal cord gray matter after acute spinal cord injury. The mechanisms maybe that Salvia increases the active of ChAT to restore the motor function of spinal cord gray matter, and increases the active of associated protein in synaptic to promote the transfer of nerve impulses. |
PDF Full Text Request