Salvia Miltiorrhiza Antagonizes Spinal Cord Ischemic/Reperfusion Injury By Interfering IL-1β,ICAM-1,MPO In Inflammatory Pathway

Objective:To investigate the effect of Salvia miltiorrhiza on the expression of intercellular adhesion molecule-1 and the activity of interleukin-1β,myeloperoxidase during Spinal Cord Ischemic/Reperfusion Injury and explore the protective mechanism of Salvia miltiorrhiza on the Spinal Cord Ischemic/Reperfusion Injury.Methods:A total of 140 Sprague Dawley rats,aged 60 days,weighing(250±10)g,of clean grade and either gender were randomly divied into four groups:sham-surgery(n=35), model(n=35), Salvia miltiorrhiza(n=35),and Tanshinone(n=35) groups. Prior to I/R model establishment, rats of the Salvia miltiorrhiza group rats were intraperitoneally injected with Salvia miltiorrhiza and rats of the Tanshinone group were intraperitoneally injected with sulfotanshinone sodium.Rats in the model, Salvia miltiorrhiza and Tanshinone group were established the spinal I/R injury model according to a modified version of Zivin method.Rats in the sham-surgery were treated with the same operation, but the abdominal aorta were not ligated.Samles from all group were harvested,respectively,at 30-minute post-ischemia,and 0.5,1,4,8,12-hour post-reperfusion,and take out the spinal cord,then observed the expression of ICAM-1 and the activity of IL-1β,MPO.Results:1,In the sham-surgery group, IL-1β,ICAM-1,MPO were not significantly change in 12 hours, and there were not ICAM-1-positive blood vessels.2,In the model,Salvia miltiorrhiza and Tanshinone group, IL-1βbegan were increased at 0.5-hour post-reperfusion, and keep increasing in 12 hours.IL-1βactivity of the Salvia miltiorrhiza and Tanshinone group were significantly less than the model group at 4,8,and 12-hours post-reperfusion(p<0.05). IL-1βactivity of the Tanshinone group was significantly less than the Salvia miltiorrhiza group at 8 and 12-hour post-reperfusion(8h p<0.05,12h p<0.01).3,When detecting ICAM-1 by ELISA,in the model,Salvia miltiorrhiza and Tanshinone group, ICAM-1 were increased at 4-hour post-reperfusion,and keep increasing in 12 hours.ICAM-1 of the Salvia miltiorrhiza and Tanshinone group was significantly less than model group at 4,8 and12-hour post-reperfusion(p<0.05).ICAM-1 of the Tanshinone group was significantly less than Salvia miltiorrhiza group at 8 and 12-hour post-reperfusion(8h p<0.05,12h p<0.01).4,When detecting ICAM-1 by Immunohistochemistry,in the model,Salvia miltiorrhiza and Tanshinone group,ICAM-1-positive blood vessels were appeared at 4-hour post-reperfusion,and keep increasing in 12 hours.ICAM-1-positive blood vessels of the Salvia miltiorrhiza and Tanshinone group was significantly less than model group at 4,8 and12-hour post-reperfusion(p<0.05).ICAM-1-positive blood vessels of the Tanshinone group was significantly less than Salvia miltiorrhiza group at 8 and 12-hour post-reperfusion(8h p<0.05,12h p<0.01).5,In the model,Salvia miltiorrhiza and Tanshinone group,MPO were increased at 0.5-hour post-reperfusion, and keep increasing in 12 hours.IL-1βactivity of the Salvia miltiorrhiza and Tanshinone group was significantly less than the model group at 4,8,12-hour post-reperfusion(p<0.05).MPO of the Salvia miltiorrhiza and Tanshinone group had not significant difference(p>0.05).Conclusions:1,Making spinal cord ischemic reperfusion injury model with Zivin'method have the characters of stability,and esay to operating and repeating.2,Salvia miltiorrhiza and Tanshinone can reduce activity of IL-1β,expression of ICAM-1 and neutrophil infiltration of spinal cord in spinal cord ischemia-reperfusion injury. The function of Tanshinone reducing IL-1βactivity and the expression of ICAM-1 in spinal cord ischemia-reperfusion injury was better than Salvia miltiorrhiza.3,Salvia miltiorrhiza can improve Spinal Cord Ischemic/Reperfusion Injury.